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RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle

文献类型： 外文期刊

作者： Li, Guanghui 1 ; Yang, Runjun 1 ; Lu, Xin 1 ; Liu, Yue 1 ; He, Wei 1 ; Li, Yue 1 ; Yu, Haibin 1 ; Qin, Lihong 3 ; Cao, Yang 3 ; Zhao, Zhihui 1 ; Fang, Xibi 1 ;

作者机构： 1.Jilin Univ, Coll Anim Sci, Changchun 130062, Peoples R China

2.Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524000, Peoples R China

3.Jilin Acad Agr Sci, Branch Anim Husb, Changchun 130062, Peoples R China

关键词： beef cattle; RNA sequencing; differentially expressed genes; meat quality traits; fat metabolism

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Meat quality has a close relationship with fat and connective tissue; therefore, screening and identifying functional genes related to lipid metabolism is essential for the production of high-grade beef. The transcriptomes of the Longissimus dorsi muscle in Wagyu and Chinese Red Steppe cattle, breeds with significant differences in meat quality and intramuscular fat deposition, were analyzed using RNA-seq to screen for candidate genes associated with beef quality traits. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the 388 differentially expressed genes (DEGs) were involved in biological processes such as short-chain fatty acid metabolism, regulation of fatty acid transport and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. In addition, crystallin alpha B (CRYAB), ankyrin repeat domain 2 (ANKRD2), aldehyde dehydrogenase 9 family member A1 (ALDH9A1) and enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (EHHADH) were investigated for their effects on intracellular triglyceride and fatty acid content and their regulatory effects on genes in lipogenesis and fatty acid metabolism pathways. This study generated a dataset from transcriptome profiling of two cattle breeds, with differing capacities for fat-deposition in the muscle, and revealed molecular evidence that CRYAB, ANKRD2, ALDH9A1 and EHHADH are related to fat metabolism in bovine fetal fibroblasts (BFFs). The results provide potential functional genes for maker-assisted selection and molecular breeding to improve meat quality traits in beef cattle.

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