Illumina Sequencing Analysis of the Microbial Community in the Gastrointestinal Tract of Chinese Mitten Crab (Eriocheir sinensis)
文献类型： 外文期刊
作者： Zhou, Yanfeng 1 ; Cheng, Yanfen 2 ; Jin, Bei 1 ; You, Yang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
2.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
关键词： Chinese mitten crab; Microbial community; Miseq; PICRUSt; 16S rRNA gene
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2019 年 21 卷 4 期
页码：
收录情况： SCI
摘要： The Chinese mitten crab (big sluice crab, Eriocheir sinensis) is one of the most economically important aquatic species in China. The present study was aimed to investigate the microbial community and their potential functions in the gastrointestinal (GI) tract of the crab. The whole gut was collected for DNA extraction and subsequent Illumina MiSeq sequencing of the bacterial 16S rRNA gene. A total of 786,410 valid sequences and 752 operational taxonomic units (OTUs) were obtained. The number of OTUs observed in the GI tract of the crab was 190.04 +/- 5.59 and the Shannon index was 4.31 +/- 0.12. The dominant phyla included Tenericutes, Bacteroidetes, Proteobacteria and Firmicutes. The predominant genera were unassigned and supposedly belong to the class of Mollicutes, which accounted for 41.64% in average. The dominant genera also included five assigned genera, which were Bacteroides, Vibrio, Shewanella, Candidatus Hepatoplasma, Dysgonomonas and four unassinged genera, which belong to the classes of Mollicutes, Alphaproteobacteria, Bacteroidia, and Gammaproteobacteria. No significant difference was observed in microbial community between male and female crabs. Functional prediction with PICRUSt suggested that the microbes in the GI tract of the crab were mainly involved in replication and repair, carbohydrate and amino acid metabolism, membrane transport, and translation. The microbes in the GI tract of the crab may play key roles in the metabolism of nutrients. Further studies are needed to control the microbial community to improve the feed efficiency of the Chinese mitten crab. (C) 2019 Friends Science Publishers
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