Preparative isolation and purification of zearalenone from rice culture by combined use of macroporous resin column and high-speed counter-current chromatography
文献类型： 外文期刊
作者： Wang, Gang 1 ; Chen, Wenhua 2 ; Hu, Junqiang 1 ; Fan, Ben 3 ; Shi, Jianrong 1 ; Xu, Jianhong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Collaborat Innovat Ctr Modern Grain Circulat & Sa, Inst Food Safety & Nutr,Minist Agr & Rural Affair, Minist Agr & Rural Affairs,Key Lab Agroprod Safet, Nanjing, Jiangsu, Peoples R China
2.Shandong Agr Univ, Coll Life Sci, Dept Microbiol, Key Lab Agr Microbiol, Tai An, Shandong, Peoples R China
3.Nanjing Forestry Univ, Coll Forestry, Nanjing, Jiangsu, Peoples R China
关键词： Zearalenone; Preparative purification; Macroporous resin; High-speed counter-current chromatography
期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）
ISSN： 1570-0232
年卷期： 2019 年 1110 卷
页码：
收录情况： SCI
摘要： Zearalenone is one of the most harmful mycotoxins found in grains and there is a large demand for zearalenone substrate for research purposes. A new separation method was developed for the preparative purification of zearalenone from rice culture of Fusarium graminearum by utilizing macroporous resin column combined with high-speed counter-current chromatography. Zearalenone was adsorpted on XAD-2 resin at 25 degrees C, neutral pH and a feed flow of 4 BV/h, followed by dynamic desorption by 60% ethanol solution. Further purification was achieved by high-speed counter-current chromatography using an optimized biphasic solvent system. A total of 267 mg of zearalenone crystal was obtained in one single run from 4.2 g of crude extract. The purity of the final product was 98.9% and the total recovery yield of zearalenone in this study was 73.9%. This dual-step purification procedure provided an effective way to obtain the costly mycotoxin for both toxicological and detoxification studies on zearalenone.
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