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Thin-layer MoS2 and thionin composite-based electrochemical sensing platform for rapid and sensitive detection of zearalenone in human biofluids

文献类型： 外文期刊

作者： Jiang, Keqiu 1 ; Nie, Dongxia 2 ; Huang, Qingwen 1 ; Fan, Kai 2 ; Tang, Zhanmin 2 ; Wu, Yongjiang 1 ; Han, Zheng 2 ;

作者机构： 1.Zhejiang Univ, Coll Pharmaceut Sci, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China

2.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Shanghai Key Lab Protected Hort Technol, Minist Agr,Lab Qual & Safety Risk Assessment Agro, Shanghai 201403, Peoples R China

关键词： Biofluid; Immunosensor; Platinum nanoparticle-modified antibody; Thin-layer MoS2 and thionin composite; Zearalenone

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN： 0956-5663

年卷期： 2019 年 130 卷

页码：

收录情况： SCI

摘要： In this study, a facile electrochemical sensing platform based on thin-layer MoS2 and thionin (MoS2-Thi) composites was constructed for the sensitive and rapid detection of zearalenone (ZEA) in human biofluids. MoS2-Thi composites with a thin-layer MoS2 structure and large specific surface area were synthesized via a simple ultrasound exfoliation method. To further enhance the performance of the platform but without increasing the complexity of the fabrication process, ZEA antibodies were modified with Pt nanoparticles (Pt NPs) using an environmentally friendly method, where they had a much wider linear range and four times higher sensitivity compared with the original ZEA monoclonal antibody (ZEA-MAb). Under the optimal conditions, the electrochemical responses of the MoS2-Thi composite-based immunosensor were linear (R-2 = 0.9915) when the ZEA concentrations ranged from 0.01 to 50 ng mL(-1). The proposed immunosensor could detect ZEA at concentrations as low as 0.005 ng mL(-1). with excellent selectivity. The immunosensor exhibited acceptable stability with high reproducibility and accuracy, and it was applied successfully for determining ZEA in human plasma and urine samples. Our findings indicated that the proposed MoS2-Thi composite-based electrochemical sensing platform provides a new approach for the rapid and sensitive bioanalysis of ZEA, and it may also be extended to other mycotoxins for multiplex detection.

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