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Influence of Nitrogen Levels on Nutrient Transporters and Regulators of Protein Synthesis in Small Intestinal Enterocytes of Piglets

文献类型： 外文期刊

作者： Tian, Zhimei 1 ; Ma, Xianyong 1 ; Deng, Dun 1 ; Cui, Yiyan 1 ; Chen, Weidong 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Sci, Minist Agr, Key Lab Anim Nutr & Feed Sci South China, Guangzhou 510640, Guangdong, Peoples R China

3.State Key Lab Livestock & Poultry Breeding, Guangzhou 510640, Guangdong, Peoples R China

4.Guangdong Acad Agr Sci, Guangdong Engn Technol Res Ctr Anim Meat Qual & S, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China

关键词： crude protein; transporter; protein synthesis; small intestine; IPEC-1 cells

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 10 期

页码：

收录情况： SCI

摘要： To investigate effects of dietary nitrogen level on nutrient absorption and utilization in small intestinal enterocyte of piglets, weaned piglets were fed for 10 days with diets containing 20%, 17%, or 14% crude protein (CP) with supplementation to meet requirements for essential amino acids in vivo, and IPEC-1 cells were cultured with different nitrogen levels (NL) in a culture medium (70%, 85%, and 100%) in vitro by monocultured and cocultured intestinal porcine epithelial cells (IPEC-1) and human gastric epithelial cells (GES-1). The results showed the following: (1) In animal trial, decreased dietary CP reduced transcript abundance of nutrient transporters like CAT1, PepT1, GLUT2, and SGLT-1 in jejunal mucosa (0.09 +/- 0.03, P < 0.0001; 0.40 +/- 0.04, P = 0.0087; 0.20 +/- 0.07, P = 0.0003; 0.35 +/- 0.02, P = 0.0001), but 17% CP diet did not affect jejunal protein synthesis. (2) The transcript abundance of nutrient transporters displayed similarly effective tendency in jejunal mucosa and cocultured IPEC-1 rather than that in monocultured IPEC-1. (3) Decreased nitrogen levels reduced expressive abundance of PI3K, Class 3 PI3K, TSC2, and 4E-BP1 in monocultured IPEC-1, but 85% nitrogen level did not affect expressive abundance of PI3K, TSC2, mTORC1, 4E-BP1, and S6K1 in cocultured IPEC-1. In general, decreased 3% CP or 15% nitrogen level reduced relative transcript expression of nutrient transporters, but did not affect protein synthesis in jejunal mucosa and cocultured IPEC-1. Therefore, decreased 3% dietary CP increased utilized and synthetic efficiency of nitrogen resource in small intestine and was beneficial in saving the dietary nitrogen resource.

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