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Gene characterization and expression pattern of Mx and Viperin genes in Dabry's sturgeon Acipenser dabryanus

文献类型： 外文期刊

作者： Zhang, Shuhuan 1 ; Lv, Xiaoyan 2 ; Deng, Dan 2 ; Zeng, Ling 2 ; Huang, Wei 2 ; Tang, Dongdong 2 ; Zhang, Lifeng 2 ; Xu, Q 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan, Hubei, Peoples R China

2.Yangtze Univ, Sch Anim Sci, Jingzhou, Peoples R China

关键词： Dabry's sturgeon Acipenser dabryanus; expression pattern; gene characterization; Mx; Viperin

期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）

ISSN： 0175-8659

年卷期： 2019 年 35 卷 2 期

页码：

收录情况： SCI

摘要： Mx and Viperin are important interferon-stimulated genes that mediate the antiviral immune response. In this study, we cloned the Mx and viperin genes from Dabry's sturgeon (Acipenser dabryanus). The Mx cDNA sequence contained an open reading frame (ORF) of 1,449 nucleotides, encoding a putative protein of 392 aa, which is significantly shorter than other animal Mx proteins. Although the similarity and identity were low between sturgeon Mx and other animal Mx proteins, sturgeon Mx contains the conserved tripartite GTP binding motif and a dynamin family signature. The sturgeon Mx gene contains eight exons split by seven introns. The sturgeon viperin cDNA sequence contained an ORF of 1,047 bp encoding a putative protein of 349 aa, which is relatively well conserved among species. Sturgeon viperin proteins show 82% similarity with those of Xiphophorus maculatus platyfish and Poecilia formosa Amazon molly. The sturgeon viperin gene has a six exon/five intron structure with the same size of second, third, fourth, and fifth exons between different species. The expression of Mx and viperin was detectable in all tissues examined, with the highest expression in skin for Mx and in peripheral blood for viperin. After mock infection using polyinosine-polycytidylic acid, Mx and viperin showed significantly upregulated expression in primary spleen leukocytes from 3 hr to 72 hr. Lipopolysaccharide could also induce their expression. These results suggested Mx and Viperin could play a vital antiviral role in the innate immune system of Dabry's sturgeon.

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