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-Glucanase specific expression in the intestine of transgenic pigs

文献类型： 外文期刊

作者： Guan, Li-zeng 1 ; Zhao, Shuai 2 ; Shu, Gang 2 ; Jiang, Qing-yan 2 ; Cai, Geng-yuan 2 ; Wu, Zhen-fang 2 ; Xi, Qian-yun; 1 ;

作者机构： 1.Linyi Univ, Coll Agr & Forestry Sci, Shuangling Rd, Linyi, Shandong, Peoples R China

2.South China Agr Univ, Natl Engn Res Ctr Breeding Swine Ind, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Coll Anim Sci,SCAU Alltech Res Joint Alliance, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China

关键词： -Glucanase; Intestine; Transgenic pigs

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN： 0962-8819

年卷期： 2019 年 28 卷 2 期

页码：

收录情况： SCI

摘要： Producing heterologous enzymes in the animal digestive tract to improve feed utilization rate is a new research strategy by transgenic technology. In this study, transgenic pigs specifically expressing -glucanase gene in the intestine were successfully produced by somatic cell nuclear transfer technology in order to improve digestibility of dietary -glucan and absorption of nutrients. The -glucanase activity in the intestinal juice of 4 transgenic pigs was found to be 8.59 +/- 2.49U/mL. The feeding trial results showed that the crude protein digestion of 4 transgenic pigs was significantly increased compared with that of the non-transgenic pigs. In order to investigate the inheritance of the transgene, 7 G(1) transgenic pigs were successfully obtained. The -glucanase activity in the intestinal juice of 7 G(1) transgenic pigs was found to be 2.35 +/- 0.72U/mL. The feeding trial results showed the crude protein digestion and crude fat digestion were significantly higher in 7 G1 transgenic pigs than in non-transgenic pigs. Taken together, our study demonstrated that the foreign -glucanase expressing in the intestine of the transgenic pigs could reduce the anti-nutritional effect of -glucans in feed. In addition, -glucanase gene could be inherited to the offsprings and maintain its physiological function. It is a promising approach to improve feed utilization by producing transgenic animals.

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