Isolation, characterization and germline chimera preparation of primordial germ cells from the Chinese Meiling chicken
文献类型： 外文期刊
作者： Yu, Fuxian 1 ; Zhu, Zhiwei 1 ; Chen, Xiaoyu 1 ; Huang, Jing 1 ; Jia, Ruoxin 1 ; Pan, Jianzhi 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Chinese Meiling chicken; primordial germ cells; separation and purification; germline chimera preparation
期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）
ISSN： 0032-5791
年卷期： 2019 年 98 卷 2 期
页码：
收录情况： SCI
摘要： Primordial germ cells (PGCs) are precursors of germline cells that can generate sperm and eggs in adults, making them promising tools for transgenic animal preparation and germplasm preservation, especially in avians. In this study, we purified the PGCs from circulating embryonic blood of Chinese Meiling chickens using Nycodenz density centrifugation, and characterized them by alkaline phosphatase (AKP) staining, periodic acid-Schiff (PAS) staining and stage-specific embryonic antigen-1 (SSEA-1) immunostaining and PGC-specific gene amplification. The purified PGCs were also labeled with PKH26 and transferred into donor chicken embryos at the Hamburger-Hamilton (HH) stage 14 to 16, and cells with red fluorescence were observed in the gonads of 8-d-old embryos. When using about 200 PGCs isolated from Chinese Meiling chickens, microinjection into the dorsal aortas of recipient chickens with white feathers at stage HH14 to 16 resulted in germline chimeras that hatched and attained sexual maturity. The frequency of donor-derived yellow-feathered offspring from germline chimeric chickens was 12.6 +/- 2.6% after mating with the white-feathered chickens. These results demonstrate that we had successfully purified the PGCs from the Chinese Meiling chicken. These germline cells could be used to preserve Chinese Meiling chickens.
- 相关文献
作者其他论文 更多>>
-
Statistical Analysis of Reproductive Traits in Jinwu Pig and Identification of Genome-Wide Association Loci
作者：Chen, Wenduo;Zhao, Ayong;Chen, Wenduo;Zhao, Ayong;Pan, Jianzhi;Tan, Kai;Zhu, Zhiwei;Zhang, Liang;Yu, Fuxian;Liu, Renhu;Huang, Jing;Zhong, Liepeng
关键词：reproductive traits; genome-wide association analysis; Jinwu pig; SNPs
-
Recombinant nanobody expressed by transgenic chimeric chickens eliminated vertical transmission of avian leukosis virus subgroup J
作者：Liang, Jiahua;Qin, Ke;Zhao, Yingying;Han, Ruiying;Wang, Jinhan;Liu, Jiahan;Lu, Huicheng;Wang, Xiaoli;Tang, Xiaochuan;Xiao, Ning;Yu, Fuxian
关键词：Avian leukosis virus subgroup J; Nanobody; Adenovirus; Transgenic chimeric chickens; Vertical transmission
-
CRISPR/Cas9-meditated gene knockout in pigs proves that LGALS12 deficiency suppresses the proliferation and differentiation of porcine adipocytes
作者：Wu, Wenjing;Yin, Yajun;Zhang, Jin;Huang, Jing;Pan, Jianzhi;Yang, Ruifei;Li, Qiuyan;Zhang, Jin
关键词：LGALS12; CRISPR/Cas9; Knockout; Pig fat
-
The Influence of Increasing Roughage Content in the Diet on the Growth Performance and Intestinal Flora of Jinwu and Duroc x Landrace x Yorkshire Pigs
作者：Xu, Gaili;Chen, Wenduo;Zhao, Ayong;Xu, Gaili;Chen, Wenduo;Zhao, Ayong;Huang, Jing;Pan, Jianzhi;Yu, Fuxian
关键词：roughage diet; Jinwu pig; Duroc x Landrace x Yorkshire pig; roughage tolerance; gut microbiota
-
Characterization of Zizania latifolia polysaccharide-corn starch composite films and their application in the postharvest preservation of strawberries
作者：Huang, Jing;Wang, Yuhua;Wu, Weijie;Niu, Ben;Fang, Xiangjun;Chen, Hangjun;Gao, Haiyan
关键词：Polysaccharide extraction; Antioxidant film; Storage; Bio-based polymers; Shelf-life extension
-
Sulfur dioxide maintains storage quality of table grape (Vitis vinifera cv 'Kyoho') by altering cuticular wax composition after simulated transportation
作者：Li, Zhenbiao;Huang, Jing;Yang, Mingyi;Li, Dong;Xu, Yanqun;Li, Li;Luo, Zisheng;Li, Zhenbiao;Huang, Jing;Yang, Mingyi;Li, Dong;Li, Li;Luo, Zisheng;Chen, Hangjun;Xu, Yanqun;Li, Li;Luo, Zisheng;Chen, Jianye;Wu, Bin;Wu, Bin
关键词：Cuticular wax; Table grape; Simulated transportation; Quality; SO2
-
Protective efficacy of Toxoplasma gondii GRA12 or GRA7 recombinant proteins encapsulated in PLGA nanoparticles against acute Toxoplasma gondii infection in mice
作者：Sun, Hong-chao;Fu, Yuan;Shi, Tuan-yuan;Deng, Pu-ming;Deng, Jin-hua;Qi, Meng;Xie, Rong-hui;Huang, Jing
关键词：GRA7; GRA12; nanoparticles; PLGA; Toxoplasma gondii; vaccine
