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MicroRNA-224 impairs adipogenic differentiation of bovine preadipocytes by targeting LPL

文献类型： 外文期刊

作者： Zhang, Yingying 2 ; Wang, Yaning 1 ; Wang, Hongbao 1 ; Ma, Xueyao 1 ; Zan, Linsen 1 ;

作者机构： 1.Northwest A&F Univ, Natl Beef Cattle Improvement Ctr, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China

2.Shanghai Acad Agr Sci, Anim Husb & Vet Res Inst, Shanghai, Peoples R China

关键词： IMF deposition; Adipogenic differentiation; miR-224; Bovine preadipocytes; LPL

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN： 0890-8508

年卷期： 2019 年 44 卷

页码：

收录情况： SCI

摘要： In this study, the potential functions of miR-224 in regulating adipogenic differentiation were explored in bovine preadipocytes. Comparative transcriptome analysis between castrated male cattle with increased intramuscular fat (IMF) and intact male cattle revealed that miR-224 and LPL were abnormally expressed, correlating negatively, and LPL was a predicted target of miR-224. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-224 and LPL. When miR-224 was either overexpressed or silenced, qRT-PCR showed a negative regulatory effect on LPL. mRNA expression levels of the fat-formationrelated biomarkers C/EBP alpha, C/EBP beta, PPAR gamma, FASN and PLIN1 decreased when miR-224 was overexpressed, while the opposite effect occurred and adipogenic differentiation followed when miR-224 was inhibited. Oil Red O staining. Triglyceride (TG) levels and immunostaining revealed that the accumulation of lipid droplets decreased or increased accordingly. Taken together, our data demonstrated that miR-224 regulated the adipogenic differentiation of bovine preadipocytes by targeting LPL. This provides insight into the molecular basis of IMF deposition in beef cattle.

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