Molecular identification and function analysis of bactericidal permeability-increasing protein/LPS-binding protein 1 (BPI/LBP1) from turbot (Scophthalmus maximus)
文献类型： 外文期刊
作者： Zhou, Shun 1 ; Jiang, Guangpeng 1 ; Zhu, Ying 3 ; Liu, Lanhao 1 ; Liu, Danyang 1 ; Diao, Jing 2 ; Liu, Hongjun 2 ; Xiu, Y 1 ;
作者机构： 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Shandong, Peoples R China
2.Marine Biol Inst Shandong Prov, Shandong Key Lab Dis Control Mariculture, Qingdao 266104, Shandong, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
关键词： Bactericidal permeability-increasing protein (BPI); lipopolysaccharide-binding protein (LBP); Scophthabnus maximus; Antibacterial activity; Immune response
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2019 年 87 卷
页码：
收录情况： SCI
摘要： Bactericidal permeability increasing protein (BPI) and lipopolysaccharide-binding protein (LBP) play important roles in host antimicrobial defense. In the present study, we identified one isoform of BPI/LBP gene from turbot (Scophthabnus maximus), designated as SmBPI/LBP1. The full-length cDNA sequence of SmBPI/LBP1 was 1826 bp, which encoding one secreted protein with 480 amino acid residues. Structurally, the SmBPI/LBP1 showed high similarity to its homologs from other vertebrates or invertebrates, which all contained a signal peptide, a BPI/LBP/CETP N-terminal with a LPS-binding domain, and a BPI/LBP/CETP C-terminal domain. The deduced amino acid sequences of SmBPI/LBP1 shared significant similarity to BPI/LBP of Seriola lalandi dorsalis (71%) and Paralichthys olivaceus (69%). Phylogentic analysis further supported that SmBPI/LBP1 act as a new member of vertebrate BPI/LBP family. SmBPI/LBP1 was ubiquitously expressed in all tested tissues, with the highest expression level in spleen tissue. The mRNA expression of SmBPI/LBP1 in spleen and kidney were significantly up-regulated after Vibrio vulnificus challenge. Finally, the recombinant SmBPI/LBP1 showed high affinity to lipopolysaccharide, followed by peptidoglycan and lipoteichoic acid, which is the ubiquitous component of Gram-negative or Gram-positive bacteria. These results indicated that SmBPI/LBP1 probably played important roles in immune response against bacteria infection.
- 相关文献
作者其他论文 更多>>
-
Development and Evaluation of a Rapid Visualisation Detection Method for Ameson portunus Based on RPA-LFD
作者：Pang, Yunfei;Song, Jiaxue;Jiang, Lirong;Lin, Yiping;Xiu, Yunji;Xiu, Yunji
关键词：
Ameson portunus ; lateral flow dipstick; rapid detection; recombinase polymerase amplification; RPA-LFD -
Population Pharmacokinetics of Enrofloxacin in Ctenopharyngodon idella Based on the Sparse Sampling Method and a Nonlinear Mixed Effect Model Following Intravenous and Oral Administration
作者：Xu, Ning;Zhang, Huan;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Dong, Jing;Ai, Xiaohui;Xu, Ning;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Dong, Jing;Ai, Xiaohui;Zhang, Huan;Ding, Yongzhen
关键词：bioavailability; enrofloxacin; grass carp; population pharmacokinetics
-
Population Pharmacokinetics of Enrofloxacin in Micropterus salmoides Based on a Nonlinear Mixed Effect Model After Intravenous and Oral Administration
作者：Xu, Ning;Zhou, Shun;Dong, Jing;Li, Jiangtao;Ai, Xiaohui;Xu, Ning;Ding, Yongzhen
关键词：enrofloxacin; population pharmacokinetics; statistical approaches; largemouth bass; body weight
-
Preclinical Evaluation of Fenbendazole for Controlling Gyrodactylus kobayashii (Monogenea, Gyrodactylidae) in Goldfish: Dose Optimization and Safety Assessment
作者：Dong, Jing;Li, Jiangtao;Liu, Yongtao;Yang, Qiuhong;Xu, Ning;Ai, Xiaohui;Zhou, Shun;Dong, Jing
关键词：preclinical study; fenbendazole; monogenean; anthelmintic treatment; aquaculture
-
Novel Insights into the Therapeutic Effect of Amentoflavone Against Aeromonas hydrophila Infection by Blocking the Activity of Aerolysin
作者：Dong, Jing;Li, Shengping;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Xu, Ning;Yang, Yibin;Ai, Xiaohui;Li, Shengping;Cheng, Bo
关键词：
Aeromonas hydrophila ; aerolysin; amentoflavone; molecular dynamics; anti-infective -
Integrated Metabolomic and Microbial Analysis of Quality Dynamics in Channel Catfish (Ictalurus punctatus) Under Refrigerated and Frozen Storage
作者：Xia, Liwei;Chen, Shengao;Zhou, Shun;Lian, Kaiqi
关键词：channel catfish; storage; lipid oxidation; metabolomics; microbiomics
-
Turmeric Oil Interferes with Quorum Sensing as an Alternative Approach to Control Aeromonas hydrophila Infection in Aquaculture
作者：Dong, Jing;Tong, Jian;Li, Shengping;Ma, Xinwei;Yang, Qiuhong;Liu, Yongtao;Zhou, Shun;Ai, Xiaohui;Ma, Xinwei;Shi, Xizhi
关键词：turmeric oil; aquaculture;
Aeromonas hydrophila ; quorum sensing; anti-infective
