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Calcium-Sensing Receptor Arbitrates Hypoxia-Induced Proliferation of Pulmonary Artery Smooth Muscle Cells via the G Protein-PLC-IP3 Pathway

文献类型： 外文期刊

作者： Zhang, Hui 1 ; Chang, Zhenyu 3 ; Mehmood, Khalid 4 ; Yang, Keli 1 ; Liu, Zewen 1 ; Duan, Zhengying 1 ; Yuan, Fangyan 1 ;

作者机构： 1.Hubei Acad Agr Sci, Anim Husb & Vet Inst, Wuhan 430064, Hubei, Peoples R China

2.Minist Agr, Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430070, Hubei, Peoples R China

3.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China

4.Islamia Univ Bahawalpur, Univ Coll Vet & Anim Sci, Bahawalpur 63100, Pakistan

5.Wuhan Gen Team Agr Comprehens Law Enforcement Sup, Wuhan 430014, Hubei, Peoples R China

6.Univ Vet & Anim Sci, Dept Clin Med & Surg, Lahore 54600, Pakistan

关键词： pulmonary arterial hypertension; calcium-sensing receptor; pulmonary artery smooth muscle cells; hypoxia; chickens

期刊名称：CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION （ 影响因子：1.807； 五年影响因子：2.333 ）

ISSN： 1045-4403

年卷期： 2019 年 29 卷 1 期

页码：

收录情况： SCI

摘要： Pulmonary arterial hypotension (PAH), also known as broilers ascites syndrome, is characterized by hypoxia, pulmonary artery pressure, and right heart failure. However, less information is available about the molecular mechanisms of PAH. We evaluated the mediation of calcium-sensing receptor by inducing hypoxia for the possible proliferation of pulmonary artery smooth muscle cells via the G protein pathway. For this purpose, we used an in vitro trial of chicken cell culture and continued our results by using immunohistochemistry, immunofluorescence staining, quantitative real-time polymerase chain reaction assay, and Western blotting analysis. Our results showed that the mRNA and protein expression levels of calcium-sensing receptor (CaSR) were significantly upregulated in cells when co-incubated with CaCl2. However, the levels of mRNA and protein were obviously decreased when supplemented with blocking agents (NiCl2, 2-APB, and D609). Furthermore, the experimentally induced hypoxia also upregulated the expression of CaSR gene as compared to CaSR gene expression in control cells. Together, these results indicate that hypoxia plays an important role in the expression of CaSR gene in pulmonary artery smooth muscle cells and reveals new targets for the CaSR excited hypothesis to prevent and control PAH in chickens.

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