Gene expression and phenoloxidase activities of hemocyanin isoforms in response to pathogen infections in abalone Haliotis diversicolor
文献类型： 外文期刊
作者： Yao, Tuo 1 ; Zhao, Man-Man 1 ; He, Jian 3 ; Han, Tao 1 ; Peng, Wen 1 ; Zhang, Han 1 ; Wang, Jiang-Yong 1 ; Jiang, Jing-Zh 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
2.Novoprot Sci Inc, Wujiang 215200, Jiangsu, Peoples R China
3.Chinese Acad Med Sci, Inst Lab Anim Sci, Beijing 100021, Peoples R China
4.Peking Union Med Coll, Beijing 100021, Peoples R China
关键词： Mollusks; Haliotis diversicolor; Innate immunity; Hemocyanin; Gene clone; Expression profile; Hemocyanin-derived peptides; Phenoloxidase; Immune defense
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2019 年 129 卷
页码：
收录情况： SCI
摘要： Hemocyanins (Hc), the main protein components of hemolymph in invertebrates, are not only involved in oxygen transport but also linked to non-specific immune responses. In this study, we used abalone (Haliotis diversicolor) Hc to study the basis of its diversified functions through gene, protein, peptides, and phenoloxidase (PO) activity levels. Three complete hemocyanin gene (HdH) sequences were cloned for the first time. By comparing the copies and location of HdH between abalone and other mollusks, we propose that Hc gene duplication and linkage is likely to be common during the evolution of mollusk respiratory proteins. We further demonstrate that all three genes could be expressed in abalone, with expression varying based on the developmental stages, tissue types, and different pathogen infections. However, HdH1 and HdH2 appear to be synthesized by the same cells by fluorescence in situ hybridization. Furthermore, the PO activity of HdH can be induced by trypsin, urea, and SDS in vitro. Viral infection can stimulate its PO activity in vivo by cleaving the protein into fragments. Consequently, we present a comprehensive study of abalone hemocyanin, providing important evidence for an indepth understanding of the physiological and immune functions of Hc in mollusks. (C) 2019 Elsevier B.V. All rights reserved.
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