ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells
文献类型： 外文期刊
作者： Qu, Xiaolu 1 ; Yan, Leyan 1 ; Guo, Rihong 1 ; Li, Hui 1 ; Shi, Zhendan 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Anim Breeding & Reprod, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing 210014, Jiangsu, Peoples R China
3.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Jilin, Peoples R China
期刊名称：OXIDATIVE MEDICINE AND CELLULAR LONGEVITY （ 影响因子：6.543； 五年影响因子：7.454 ）
ISSN： 1942-0900
年卷期： 2019 年
页码：
收录情况： SCI
摘要： LPS is a major endotoxin produced by gram-negative bacteria, and exposure to it commonly occurs in animal husbandry. Previous studies have shown that LPS infection disturbs steroidogenesis, including progesterone production, and subsequently decreases animal reproductive performance. However, little information about the underlying mechanisms is available thus far. In the present study, an in vitro-luteinized porcine granulosa cell model was used to study the underlying molecular mechanisms of LPS treatment. We found that LPS significantly inhibits progesterone production and downregulates the expressions of progesterone synthesis-associated genes (StAR, CYP11A1, and 3 beta-HSD). Furthermore, the levels of ROS were significantly increased in an LPS dose-dependent manner. Moreover, transcriptional factors GATA4 and GATA6, but not NR5A1, were significantly downregulated. Elimination of LPS-stimulated ROS by melatonin or vitamin C could restore the expressions of GATA4, GATA6, and StAR. In parallel, StAR expression was also inhibited by the knockdown of GATA4 and GATA6. Based on these data, we conclude that LPS impairs StAR expression via the ROS-induced downregulation of GATA4 and GATA6. Collectively, these findings provide new insights into the understanding of reproductive losses in animals suffering from bacterial infection and LPS exposure.
- 相关文献
作者其他论文 更多>>
-
Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene
作者：Chen, Rong;Chen, Chen;Qin, Yifei;Liu, Jie;Lei, Mingming;Zhu, Huanxi;Shi, Zhendan;Chen, Rong;Chen, Chen;Qin, Yifei;Liu, Jie;Lei, Mingming;Zhu, Huanxi;Shi, Zhendan
关键词：Goose; Anti-M & uuml;llerian hormone; Vitamin D response element
-
Kynurenine, a derivative of tryptophan, inhibits progesterone biosynthesis in porcine granulosa luteal cells through Aryl hydrocarbon receptor-mediated downregulation of GATA-binding protein 4, 6, and CCAAT/enhancer-binding protein beta†
作者：Liao, Shiying;Deng, Yanfei;Liao, Shiying;Cheng, Jinhua;Zhao, Weimin;Dai, Chaohui;Fu, Yanfeng;Li, Bixia;Li, Hui;Li, Hui
关键词：kynurenine; progesterone; granulosa luteal cell; STAR; AHR
-
The effects of post-hatch brooding temperature on the growth performance, meat quality and serum index of Pekin ducks
作者：Xu, Congcong;Sun, Dongyue;Dai, Zichun;Guo, Rihong;Shi, Zhendan;Ying, Shijia;Xu, Congcong;Sun, Dongyue;Ying, Shijia;Shi, Zhendan;Ying, Shijia
关键词：Pekin duck; Brooding temperature; Production performance; Serum indices; Meat quality
-
Effects of environmental temperature on growth performance, muscle fiber structure, fatty acid composition, and gene expression in ducks
作者：Pan, Ziyi;Xu, Congcong;Sun, Dongyue;Yang, Xingda;Guo, Rihong;Dai, Zichun;Chen, Fang;Shi, Zhendan;Ying, Shijia;Shi, Zhendan;Ying, Shijia
关键词：Ambient temperature; Duck; Muscle fiber; Fatty acids; Cell cycle
-
Effects of Different Levels of Lycium barbarum Flavonoids on Growth Performance, Immunity, Intestinal Barrier and Antioxidant Capacity of Meat Ducks
作者：Tu, Minhang;Cai, Gentan;Ma, Longfei;Wang, Tian;Wang, Chao;Yan, Leyan;Shi, Zhendan;Chen, Zhe;Yan, Leyan;Shi, Zhendan;Chen, Zhe
关键词：
Lycium barbarum flavonoids; meat duck; growth performance; intestinal function; antioxidation -
The Erk1/2-EGR1 signaling pathway is involved in lipopolysaccharide-induced transforming growth factor-beta 1 expression in mouse macrophages
作者：Zhao, Weimin;Wang, Hong;Dai, Chaohui;Li, Bixia;Fu, Yanfeng;Cheng, Jinhua;Li, Hui;Wang, Hong;Li, Hui
关键词：LPS; TGF-beta 1; Erk1/2; EGR1; Macrophage
-
An advanced cytosine base editor enabled the generation of cattle with a stop codon in the β-lactoglobulin gene
作者：Ding, Qiang;Cui, Zhaokang;Shi, Qianqian;Guo, Rihong;Cao, Shaoxian;Zhong, Jifeng;Wang, Huili;Zhang, Yan;He, Nan;Tian, Yu
关键词：beta-Lactoglobulin; Stop codon; Base editing; CBE; Cattle
