MiR-34b-5p Mediates the Proliferation and Differentiation of Myoblasts by Targeting IGFBP2
文献类型： 外文期刊
作者： Wang, Zhijun 1 ; Zhang, Xiaocui 1 ; Li, Zhenhui 1 ; Abdalla, Bahareldin Ali 1 ; Chen, Yangfeng 1 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
2.Minist Agr, Natl Local Joint Engn Res Ctr Livestock Breeding, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
3.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
关键词： miR-34b-5p; IGFBP2; skeletal muscle; myoblast; myogenesis
期刊名称：CELLS （ 影响因子：6.6； 五年影响因子：6.663 ）
ISSN：
年卷期： 2019 年 8 卷 4 期
页码：
收录情况： SCI
摘要： As key post-transcriptional regulators, microRNAs (miRNAs) play an indispensable role in skeletal muscle development. Our previous study suggested that miR-34b-5p and IGFBP2 could have a potential role in skeletal muscle growth. Our goal in this study is to explore the function and regulatory mechanism of miR-34b-5p and IGFBP2 in myogenesis. In this study, the dual-luciferase reporter assay and Western blot analysis showed that IGFBP2 is a direct target of miR-34b-5p. Flow cytometric analysis and EdU assay showed that miR-34b-5p could repress the cell cycle progression of myoblasts, and miR-34b-5p could promote the formation of myotubes by promoting the expression of MyHC. On the contrary, the overexpression of IGFBP2 significantly facilitated the proliferation of myoblasts and hampered the formation of myotubes. Together, our results indicate that miR-34b-5p could mediate the proliferation and differentiation of myoblasts by targeting IGFBP2.
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