MiR-34b-5p Mediates the Proliferation and Differentiation of Myoblasts by Targeting IGFBP2
文献类型： 外文期刊
作者： Wang, Zhijun 1 ; Zhang, Xiaocui 1 ; Li, Zhenhui 1 ; Abdalla, Bahareldin Ali 1 ; Chen, Yangfeng 1 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
2.Minist Agr, Natl Local Joint Engn Res Ctr Livestock Breeding, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
3.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
关键词： miR-34b-5p; IGFBP2; skeletal muscle; myoblast; myogenesis
期刊名称：CELLS （ 影响因子：6.6； 五年影响因子：6.663 ）
ISSN：
年卷期： 2019 年 8 卷 4 期
页码：
收录情况： SCI
摘要： As key post-transcriptional regulators, microRNAs (miRNAs) play an indispensable role in skeletal muscle development. Our previous study suggested that miR-34b-5p and IGFBP2 could have a potential role in skeletal muscle growth. Our goal in this study is to explore the function and regulatory mechanism of miR-34b-5p and IGFBP2 in myogenesis. In this study, the dual-luciferase reporter assay and Western blot analysis showed that IGFBP2 is a direct target of miR-34b-5p. Flow cytometric analysis and EdU assay showed that miR-34b-5p could repress the cell cycle progression of myoblasts, and miR-34b-5p could promote the formation of myotubes by promoting the expression of MyHC. On the contrary, the overexpression of IGFBP2 significantly facilitated the proliferation of myoblasts and hampered the formation of myotubes. Together, our results indicate that miR-34b-5p could mediate the proliferation and differentiation of myoblasts by targeting IGFBP2.
- 相关文献
作者其他论文 更多>>
-
The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics
作者：Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Luo, Wen;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua
关键词：TMEM182; Fat formation; Metabolomics; Lipidomics
-
Using multiomics to explore the weight differences between genders in Muscovy ducks
作者：Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Tian, Jinghong;Tan, Liangtian;Wei, Shenghua;Yao, Zipei;Xu, Yibin;Nie, Qinghua;Zhu, Weijian;Ji, Congliang
关键词：Muscovy duck; sexual dimorphism; growth and development; RNA-seq; 16S rRNA sequencing technology
-
Live chicken body fat measurement technology based on bio-electrical impedance
作者：Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Zuo, Jiaming;Cheng, Shangshang;Li, Zhen;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Liang, Jinping;Li, Zhenhui;Nie, Qinghua;Zhang, Dexiang;Zhang, Xiquan;Li, Hongmei;Deng, Yuelin
关键词：BIA; Electrical impedance; Fat content; Broilers
-
LncEDCH1 g.1703613 T>C regulates chicken carcass traits by targeting miR-196-2-3p
作者：Yuan, Rongshuai;Cai, Bolin;Ma, Manting;Zhao, Changbin;Xian, Yuanrong;Nie, Qinghua;Zhang, Xiquan;Zhang, Dexiang;Zhang, Dexiang
关键词：LncEDCH1; miR-196-2-3p; single nucleotide polymorphism; carcass trait; chicken
-
Weighted single-step GWAS identified candidate genes associated with carcass traits in a Chinese yellow-feathered chicken population
作者：Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Pan, Rongyang;Qi, Lin;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Pan, Rongyang;Qi, Lin;Xu, Zhenqiang;Zhang, Dexiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen
关键词：Chinese yellow-feathered chicken; weighted single-step GWAS; carcass traits; SNP
-
MicroRNA expression profile of chicken jejunum in different time points Eimeria maxima infection
作者：Jebessa, Endashaw;Bello, Semiu Folaniyi;Guo, Lijin;Nie, Qinghua;Jebessa, Endashaw;Bello, Semiu Folaniyi;Guo, Lijin;Nie, Qinghua;Jebessa, Endashaw;Bello, Semiu Folaniyi;Guo, Lijin;Nie, Qinghua;Jebessa, Endashaw;Hanotte, Olivier;Tuli, Merga Daba;Hanotte, Olivier
关键词：microRNA; expression profile; chicken jejunum; host immunity; Eimeria maxima
-
Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism
作者：Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Zhang, Zihao;Luo, Wen;Chen, Genghua;Chen, Jiahui;Ren, Tuanhui;Lin, Zetong;Zhao, Changbin;Wen, Huaqiang;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Nie, Qinghua;Zhang, Xiquan;Luo, Wen;Zhang, Zihao;Lin, Shudai;Meng, Xun;Meng, Xun
关键词：Muscle antibody array; Myoblast differentiation; Energy metabolism regulation
