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Description of a Natural Infection with Decapod Iridescent Virus 1 in Farmed Giant Freshwater Prawn, Macrobrachium rosenbergii

文献类型： 外文期刊

作者： Qiu, Liang 1 ; Chen, Xing 1 ; Zhao, Ruo-Heng 1 ; Li, Chen 1 ; Gao, Wen 1 ; Zhang, Qing-Li 1 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

2.Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Shandong, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao 266071, Shandong, Peoples R China

4.Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao 266071, Shandong, Peoples R China

5.Shanghai Ocean Univ, Shanghai 201306, Peoples R China

6.Dalian Ocean Univ, Dalian 116023, Peoples R China

关键词： DIV1; SHIV; CQIV; Macrobrachium rosenbergii; Macrobrachium nipponense; Procambarus clarkii; white head; susceptible species; viral load

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN： 1999-4915

年卷期： 2019 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Macrobrachium rosenbergii is a valuable freshwater prawn in Asian aquaculture. In recent years, a new symptom that was generally called white head has caused high mortality in M. rosenbergii farms in China. Samples of M. rosenbergii, M. nipponense, Procambarus clarkii, M. superbum, Penaeus vannamei, and Cladocera from a farm suffering from white head in Jiangsu Province were collected and analyzed in this study. Pathogen detection showed that all samples were positive for Decapod iridescent virus 1 (DIV1). Histopathological examination revealed dark eosinophilic inclusions and pyknosis in hematopoietic tissue, hepatopancreas, and gills of M. rosenbergii and M. nipponense. Blue signals of in situ digoxigenin-labeled loop-mediated isothermal amplification appeared in hematopoietic tissue, hemocytes, hepatopancreatic sinus, and antennal gland. Transmission electron microscopy of ultrathin sections showed a large number of DIV1 particles with a mean diameter about 157.9 nm. The virogenic stromata and budding virions were observed in hematopoietic cells. Quantitative detection with TaqMan probe based real-time PCR of different tissues in naturally infected M. rosenbergii showed that hematopoietic tissue contained the highest DIV1 load with a relative abundance of 25.4 +/- 16.9%. Hepatopancreas and muscle contained the lowest DIV1 loads with relative abundances of 2.44 +/- 1.24% and 2.44 +/- 2.16%, respectively. The above results verified that DIV1 is the pathogen causing white head in M. rosenbergii. M. nipponense and Pr. clarkii are also species susceptible to DIV1.

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