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Sperm cryopreservation of the noble Scallop Chlamys nobilis by a programmable freezing method: Effect of cryoprotectant

文献类型： 外文期刊

作者： Zheng, Xing 1 ; Lin, Siqi 1 ; Yang, Rui 3 ; Qin, Jianguang 4 ; Wang, Aimin 1 ; Gu, Zhifeng 1 ; Ma, Zhenhua 3 ;

作者机构： 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, 58 Renmin Ave, Haikou 570228, Hainan, Peoples R China

2.Hainan Univ, Ocean Coll, Haikou, Hainan, Peoples R China

3.Chinese Acad Fishery Sci, Trop Aquaculture Res & Dev Ctr, South China Sea Fisheries Res Inst, Sanya, Peoples R China

4.Flinders Univ S Australia, Coll Sci & Engn, Adelaide, SA, Australia

关键词： cryopreservation; cryoprotectant; programmable freezing; scallop Chlamys nobilis; sperm

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2019 年 50 卷 6 期

页码：

收录情况： SCI

摘要： A study on Chlamys nobilis sperm cryopreservation by a programmable freezing method was conducted under laboratory condition. Four cryoprotectant agents (dimethyl sulfoxide [DMSO], methanol [MET], propanediol[PG] and ethylene glycol [EG]) and four concentrations (5%, 10%, 20% and 30%) were evaluated for their ability to retain sperm motility, movement characteristics and fertility. Results showed that cryopreserved sperm total motility produced by DMSO and MET at 5%, 10% and 20% were higher than other cryoprotectant treatment groups (CPA groups), as well as rapid sperm percentage. The curvilinear (VCL) and straight line (VSL) velocity produced by DMSO at 5% significantly higher than other CPA groups (p < 0.05), while no significant differences were found for average path (VAP) velocity. The lateral head displacement (ALH) in all CPA groups was similar and without significant difference (p > 0.05), as well as the beat-cross frequency (BCF). A significant higher fertilization rate was produced in DMSO than that in MET at same concentration (p < 0.05), and no significant differences were found for differing concentrations of the same cryoprotectant (p > 0.05). Overall, 5%-20% DMSO was more suitable for Chlamys nobilis sperm programmable cryopreservation when the calcium-free Hanks' balanced salt solution was used as the extender, and 10 degrees C/min from 0 degrees C to -80 degrees C was used as freezing rate. The findings presented in this study will benefit conservation programs for Chlamys nobilis.

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