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Further characterisation of Leucocytozoon podargii in wild tawny frogmouths (Podargus strigoides) in Western Australia

文献类型： 外文期刊

作者： Jiang, Yanjie 1 ; Brice, Belinda 3 ; Mai Nguyen 4 ; Loh, Richmond 4 ; Greay, Telleasha 1 ; Adlard, Robert 5 ; Ryan, Un 1 ;

作者机构： 1.Murdoch Univ, Sch Vet & Life Sci, Murdoch, WA 6150, Australia

2.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

3.Kanyana Wildlife Rehabil Ctr, 120 Gilchrist Rd, Lesmurdie, WA 6076, Australia

4.Sustainabil & Biosecur Dept Primary Ind & Reg Dev, 3 Baron Hay Court, S Perth, WA 6151, Australia

5.Queensland Museum Network, South Brisbane, Qld 4101, Australia

关键词： Haematozoa; Leucocytozoon podargii; Gametocytes; Wild tawny frogmouth; ddPCR; Cytochrome b gene

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2019 年 118 卷 6 期

页码：

收录情况： SCI

摘要： The present study assessed the prevalence and morphology of Leucocytozoon podargii from wild tawny frogmouths (Podargus strigoides) in Western Australia (WA) and genetically characterised the cytochrome b gene (cyt b) of L. podargii in wild tawny frogmouths from WA and Queensland (QLD). The prevalence of L. podargii in wild tawny frogmouths from WA was 93.3% (14/15; 95% CI, 68.1-99.8%). The morphological characters of L. podargii from WA were similar to L. podargii from QLD: the gametocytes were round-oval shape, approximately 8-12m in diameter; the macrogametocytes were 12.4m in diameter; microgametocytes were 10.4m in diameter; and the ratio of macrogametocytes and microgametocytes was 3:2. Sequence analysis of partial cyt b gene fragments revealed that L. podargii sequences isolated from wild tawny frogmouths in WA shared the highest similarity (99.8% at nucleotide level and 100% at protein level) with L. podargii isolated from wild tawny frogmouths in QLD. The mitochondrial 18S rRNA gene of L. podargii gametocytes was quantified using droplet digital PCR (ddPCR), and the highest gametocyte load was detected in the lung. This finding corresponds to the results of the histological study. Based on the morphological and molecular studies, it was concluded that the Leucocytozoon parasite identified from wild tawny frogmouths in WA is consistent with L. podargii from wild tawny frogmouths in QLD, and the present study has genetically characterised two different L. podargii genotypes (QLD and WA) for the first time.

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