Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii
文献类型： 外文期刊
作者： Wang, Yao-Yao 1 ; Ma, Haile 1 ; Yan, Jing-Kun 1 ; Wang, Ke-Di 1 ; Yang, Yan 2 ; Wang, Wen-Han 2 ; Zhang, He-Nan 2 ;
作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
2.Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Natl Engn Res Ctr Edible Fungi, Shanghai, Peoples R China
3.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China
关键词： Phellinus baumii exopolysaccharide; Three-phase partitioning; Dimethyl carbonate
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2019 年 131 卷
页码：
收录情况： SCI
摘要： In this study, a three-phase partitioning (TPP) system with dimethyl carbonate (DMC) as organic phase and sodium citrate (SC) as salt phase was used for partitioning of exopolysaccharide (EPS), namely, EPS-D, from fermentation broth of Phellinus baumii. Results showed that the maximum extraction yield (EY) of EPS-D was 71.02% under the following modified optimal conditions: DMC to fermentation broth ratio 0.5:1.0 (v/v), SC concentration 19% (w/v), temperature 30 degrees C, and pH 4.0. EPS-D had higher EY, carbohydrate, and uronic add contents compared with the EPS, designated as EPS-T, obtained from the TPP system with t-butanol and ammonium sulfate. EPS-D and EPS-T had different chemical compositions and molecular weights; however, their preliminary chemical structures basically remained unchanged. Moreover, EPS-D exhibited stronger free radical-scavenging capability and total antioxidant capacity than EPS-T. Therefore, the TPP system with DMC as an alternative solvent for t-butanol has great potential for efficient partitioning of natural biomolecules. (C) 2019 Elsevier B.V. All rights reserved.
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