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Rapid identification of a candidate nicosulfuron sensitivity gene (Nss) in maize (Zea mays L.) via combining bulked segregant analysis and RNA-seq

文献类型： 外文期刊

作者： Liu, Xiaomin 1 ; Bi, Bo 2 ; Xu, Xian 1 ; Li, Binghua 1 ; Tian, Shengmin 1 ; Wang, Jianping 1 ; Zhang, Hui 2 ; Wang, Guiqi 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Shijiazhuang 050035, Hebei, Peoples R China

2.Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA

3.Northeast Agr Univ, Coll Agr, Harbin 150030, Heilongjiang, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2019 年 132 卷 5 期

页码：

收录情况： SCI

摘要： Key messageA candidate nicosulfuron sensitivity geneNsswas identified by combining bulked segregant analysis and RNA-seq. Multiple mutations of this gene were discovered in nicosulfuron-sensitive maize compared with the tolerant.AbstractIt has been demonstrated that variabilities exist in maize response to nicosulfuron. Two nicosulfuron-sensitive inbred lines (HB39, HB41) and two tolerant inbred lines (HB05, HB09) were identified via greenhouse and field trials. Genetic analysis indicated that the sensitivity to nicosulfuron in maize was controlled by a single, recessive gene. To precisely and rapidly map the nicosulfuron sensitivity gene (Nss), two independent F-2 segregating populations, Population A (HB41xHB09) and Population B (HB39xHB05), were constructed. By applying bulked segregant RNA-Seq (BSR-Seq), the Nss gene was, respectively, mapped on the short arm of chromosome 5 (chr5: 1.1-15.3Mb) and (chr5: 0.5-18.2Mb) using two populations, with 14.2Mb region in common. Further analysis revealed that there were 43 and 119 differentially expressed genes in the mapping intervals, with 18 genes in common. Gene annotation results showed that a cytochrome P450 gene (CYP81A9) appeared to be the candidate gene of Nss associated with nicosulfuron sensitivity in maize. Sequence analysis demonstrated that two common deletion mutations existed in the sensitive maize, which might lead to the nicosulfuron sensitivity in maize. The results will make valuable contributions to the understanding of molecular mechanism of herbicide sensitivity in maize.

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