Preparation of a novel polyethyleneimine functionalized sepharose-boronate affinity material and its application in selective enrichment of food borne pathogenic bacteria
文献类型： 外文期刊
作者： Zheng, Hongwei 1 ; Han, Feng 2 ; Lin, Hong 1 ; Cao, Limin 1 ; Pavase, Tushar Ramesh 1 ; Sui, Jianxin 1 ;
作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, Food Safety Lab, Qingdao 266003, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
关键词： Boronate affinity; Food borne pathogenic bacteria; mPCR; Pretreatment
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2019 年 294 卷
页码：
收录情况： SCI
摘要： Detection of pathogenic bacteria by polymerase chain reaction (PCR) is progressively emerging, although it is still hindered by a complex matrix, long-term bacterial enrichment and low bacterial abundance. Here, we report a novel material based on boronate affinity for recognition and enrichment of bacteria using a pre-PCR method. After optimization, the material exhibited high boronate affinity toward bacteria, with adsorption capacities of S. aureus and Salmonella spp. incubated in 0.01 M PBS (pH 7.4) at 37 degrees C for 15 min calculated as (906.60 +/- 15.73) x 10(7) cfu/g and (582.59 +/- 13.19) x 10(7) cfu/g, respectively, without any bacterial death during the binding process. The material was then applied to enrich S. aureus and Salmonella spp. from the spiked water and 25% cow milk samples followed by mPCR, which resulted in high bacterial enrichment and demonstrated great potential for selective enrichment of bacteria in food samples.
- 相关文献
作者其他论文 更多>>
-
Effects of steaming practices on the cadmium distribution in edible tissues of the swimming crabs (Portunus trituberculatus) and its health risk assessment
作者：Wu, Di;Mo, Xiao;Zhang, Xiaoying;Shi, Yongfu;Huang, Dongmei;Fang, Changling;Tang, Yunyu;Han, Feng;Tian, Liangliang;Li, Siman;Wu, Di;Mo, Xiao;Zhang, Xiaoying
关键词：Steaming; Cadmium; Portunus trituberculatus; Redistribution; Health risk; Brown meat
-
Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide
作者：Wu, Jieer;Yu, Yongxin;Wang, Yongjie;Wang, Yongjie;Han, Feng;Han, Feng
关键词：
Pseudomonas composti ; Psl EPS; Arabinose promoter; Overexpression -
Preparation and characterization of fish-derived protein gel as a potential dysphagia food: Co-mingling effects of inulin and konjac glucomannan mixtures
作者：Chen, Qian;Cao, Limin;Lin, Hong;Chen, Qian;Wu, Yanyan;Zhao, Yongqiang;Xiang, Huan;Wang, Yueqi;Wu, Yanyan;Wang, Yueqi
关键词：Marine protein; Inulin/Konjac Glucomannan; Swallowing properties; Dysphagia
-
Survey of the biogenic amines in typical foods commonly consumed from the Chinese market
作者：Zhang, Xuan;Fang, Changling;Lou, Xiaoyi;Han, Feng;Li, Siman;Shi, Yongfu;Huang, Dongmei
关键词：Biogenic amines; Fish; Cheese; Alcoholic beverage; Risk assessment
-
A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
作者：Han, Feng;Zhang, Xiaoya;Chen, Yunfei;Zhao, Haixia;Wu, Jieer;Yu, Yongxin;Wang, Yongjie;Han, Feng;Yu, Yongxin;Wang, Yongjie;Wang, Yongjie
关键词：Long gene cassette knockout; Seamless cloning; Pseudomonas; Homologous recombination
-
Comparative analysis of allergenicity and predicted linear epitopes in alpha and beta parvalbumin from turbot (Scophthalmus maximus)
作者：Li, Zhenxing;Huang, Yuhao;Li, Zhenxing;Wu, Yeting;Zhang, Ziye;Wang, Hao;Lin, Hong;Li, Yonghong;Pramod, Siddanakoppalu;Chen, Guanzhi;Zhu, Wenjia
关键词：parvalbumin; IgE binding; epitope; cross-reactivity; fish allergy
-
Pseudomonas composti isolate from oyster digestive tissue specifically binds with norovirus GII.6 via Psl extracellular polysaccharide
作者：Yu, Yongxin;Han, Feng;Zhang, Xiaoya;Chen, Yunfei;Yu, Mingxia;Wang, Yongjie;Wang, Yongjie;Yu, Yongxin;Wang, Yongjie;Han, Feng;Yu, Mingxia
关键词：Norovirus; Binding; Pseudomonas composti; Exopolysaccharide; Oyster
