Chitosan and anisodamine improve the immune efficacy of inactivated infectious spleen and kidney necrosis virus vaccine in Siniperca chuatsi
文献类型： 外文期刊
作者： Zhang, Jiacheng 1 ; Fu, Xiaozhe 3 ; Zhang, Yanqi 1 ; Zhu, Wentao 1 ; Zhou, Yong 4 ; Yuan, Gailing 1 ; Liu, Xiaoling 1 ; A 1 ;
作者机构： 1.Huazhong Agr Univ, Hubei Engn Technol Res Ctr Aquat Anim Dis Control, Dept Aquat Anim Med, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Aquat Anim Immune Technol, Minist Agr,Key Lab Fishery Drug Dev, Guangzhou 510380, Guangdong, Peoples R China
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Div Fish Dis, Wuhan 430223, Hubei, Peoples R China
5.Wuhan Chopper Fishery Biotech Co Ltd, Wuhan Acad Agr Sci, Wuhan 430207, Hubei, Peoples R China
关键词： Siniperca chuatsi; ISKNV; Chitosan; poly(I:C); Anisodamine; ims1312; Adjuvant
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2019 年 89 卷
页码：
收录情况： SCI
摘要： Siniperca chuatsi is an economically important fish in China, but infectious spleen and kidney necrosis virus (ISKNV) causes high mortality and significant economic losses. Currently, vaccination is the most promising strategy to prevent infectious diseases, while adjuvant can effectively enhance immune responses. In this study, inactivated ISKNV vaccine was prepared, then poly (I:C), chitosan, anisodamine and ims1312 were used as adjuvants to evaluate the effect on the immune responses and ISKNV replication. Chitosan could strongly boost the protection of liver and spleen tissues by pathological sections. In serum, poly (I:C) and chitosan group had protective effect on catalase, acid phosphatase, blood urea nitrogen. mRNA expressions showed these adjuvants induced the cytokines of early immune responses (TNF-alpha, Viperin) in both spleen and mesonephron by real time quantitative RT-PCR assays. Meanwhile, poly (I:C), chitosan and anisodamine were significantly improved the antiviral function and inhibited ISKNV replication. Chitosan and anisodamine played a significantly protective role in the immune protective rate test. The results indicated that all the four adjuvants are valid in the inactivated ISKNV vaccine, and chitosan is recommended preferentially. The present study provides reference for other animal vaccine adjuvants.
- 相关文献
作者其他论文 更多>>
-
Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
作者：Xue, Mingyang;Xu, Chen;Huang, Zhenyu;Meng, Yan;Jiang, Nan;Fan, Yuding;Zhou, Yong;Xue, Mingyang
关键词：
Cyprinid herpesvirus 2 ; ORF41; pyruvate dehydrogenase -
Inactivated LMBV vaccine with nano-aluminum - aluminum adjuvant provided immune protection in largemouth bass ( Micropterus Salmoides) )
作者：Luo, Xia;Ma, Baofu;Chang, Ouqin;Fu, Xiaozhe;Liang, Hongru;Niu, Yinjie;Lin, Qiang;Liu, Lihui;Li, Ningqiu;Wang, Ruoxuan;Lv, Aijun
关键词：Largemouth bass; LMBV; Inactivated vaccine; Nano-aluminum adjuvant; Potentiation effects
-
Identification of a novel circovirus associated with turbot (Scophthalmus maximus) acute hemorrhage disease
作者：Wang, Huilin;Huang, Zhihui;Li, Jie;Jiang, Jingzhe;Xu, Liming;Zhou, Yong;Qin, Qiwei;Wei, Jingguang;Wang, Qiyao;Xiao, Zhizhong;Li, Jie
关键词：Turbot; Turbot acute hemorrhage disease; Circovirus; Pathogen
-
Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus
作者：Liu, Yisha;Xue, Mingyang;Xu, Chen;Zhou, Yong;Jiang, Nan;Meng, Yan;Li, Yiqun;Huang, Zhenyu;Liu, Wenzhi;Li, Zhong;Fan, Yuding;Liu, Yisha;Zhong, Qiwang
关键词：Arctigenin; Rhabdovirus; Monopterus albus; Antiviral
-
Viperin and Its Effect on SVCV Replication in Common Carp, Cyprinus carpio
作者：Meng, Yan;Hu, Xi;Jiang, Nan;Fan, Yuding;Li, Yiqun;Xue, Mingyang;Xu, Chen;Liu, Wenzhi;Zhou, Yong
关键词：common carp (
Cyprinus carpio );viperin ; characteristics; antiviral activities -
Infectious Spleen and Kidney Necrosis Virus ORF093R and ORF102R Regulate Glutamate Metabolic Reprogramming to Support Virus Proliferation by Interacting with c-Myc
作者：Niu, Yinjie;Ye, Caimei;Lin, Qiang;Liang, Hongru;Luo, Xia;Ma, Baofu;Li, Ningqiu;Fu, Xiaozhe
关键词：ISKNV; c-Myc; glutamine metabolism
-
Immunological Responses and Protection in the Largemouth Bass (Microterus salmoides) Immunized with Inactivated Vaccine Against Largemouth Bass Ranavirus (LMBRaV)
作者：Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Zhou, Yong;Yang, Tao;Li, Chenyang;Yang, Tao;Zhai, Jiale;Zeng, Lingbing;Li, Yiqun;Liu, Wenzhi;Meng, Yan;Fan, Yuding;Huang, Zhenyu;Zhou, Yong;Jiang, Nan
关键词：largemouth bass; largemouth bass ranavirus; inactivated vaccine; relative percent survival rates; immune response
