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The DNA methylation level is associated with the superior growth of the hybrid fry in snakehead fish (Channa argus x Channa maculata)

文献类型： 外文期刊

作者： Ou, Mi 1 ; Mao, Huahua 1 ; Luo, Qing 1 ; Zhao, Jian 1 ; Liu, Haiyang 1 ; Zhu, Xinping 1 ; Chen, Kunci 1 ; Xu, Hongyan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resources Applicat, Guangzhou 510380, Guangdong, Peoples R China

2.Shaoxing Fisheries Tech Extens Stn, Shaoxing 312000, Peoples R China

关键词： Heterosis; DNA methylation; MSAP, Dnmt3a expression; Hybrid breeding

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2019 年 703 卷

页码：

收录情况： SCI

摘要： Hybrid vigour, or heterosis, refers to the increased productivity and growth rate of hybrid offsprings relative to the parents. Various heterosis have been well exploited in fish for fisheries. However, the molecular mechanisms underlying heterosis are largely unknown in fish. In this study, two inbred and hybrid lines between the northern snakehead (NS, Channa argus) and blotched snakehead (BS, Channa maculata) were generated. The analysis on various growth traits, including body length, head length, and body height, showed that hybrid fry obviously exhibited a spontaneous growth heterosis over the inbred. Moreover, the methylation-sensitive amplification polymorphism (MSAP) analysis revealed that the DNA methylation levels were negatively related to the body growth in all fry. Especially, the DNA methylation levels in the hybrid fry were significantly lower than those in the inbred. Additionally, qRT-PCR showed that the snakehead fish Dnmt3a mRNA was initially detectable in embryos at 12 hpf and gradually increased as developing. Intriguingly, the level of Dnmt3a mRNA expression was found to be closely correlated to the DNA methylation level in embryos/fry. The results of this study firstly demonstrated the correlations between growth heterosis, DNA methylation level and Dnmt3a mRNA expression in fish fry. The findings of this study implied that the hybrids' heterosis formation is probably accompanied by DNA methylation alterations and modulated by Dnmt3a gene in fish. This study would provide new clues for further investigations on mechanisms behind heterosis formation in fish hybrid.

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