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A Transcription Factor, OsMADS57, Regulates Long-Distance Nitrate Transport and Root Elongation

文献类型： 外文期刊

作者： Huang, Shuangjie 1 ; Liang, Zhihao 1 ; Chen, Si 1 ; Sun, Huwei 3 ; Fan, Xiaorong 1 ; Wang, Cailin 4 ; Xu, Guohua 1 ; Zhan 1 ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Key Lab Plant Nutr & Fertilizat Low Middle Reache, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China

2.Xinyang Agr & Forestry Univ, Henan Key Lab Tea Plant Comprehens Utilizat South, Xinyang 464000, Peoples R China

3.Henan Agr Univ, Coll Agron, Collaborat Innovat Ctr Henan Grain Crops, Key Lab Rice Biol Henan Prov, Zhengzhou 450002, Henan, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

ISSN： 0032-0889

年卷期： 2019 年 180 卷 2 期

页码：

收录情况： SCI

摘要： Root nitrate uptake adjusts to the plant's nitrogen demand for growth. Here, we report that OsMADS57, a MADS-box transcription factor, modulates nitrate translocation from rice (Oryza sativa) roots to shoots under low-nitrate conditions. OsMADS57 is abundantly expressed in xylem parenchyma cells of root stele and is induced by nitrate. Compared with wild-type rice plants supplied with 0.2 mM nitrate, osmads57 mutants had 31% less xylem loading of nitrate, while overexpression lines had 2-fold higher levels. Shoot-root N-15 content ratios were 40% lower in the mutants and 76% higher in the overexpression lines. Rapid NO3- root influx experiments showed that mutation of OsMADS57 did not affect root nitrate uptake. Reverse transcription quantitative PCR analysis of OsNRT2 nitrate transporter genes showed that after 5 min in 0.2 mM nitrate, only OsNRT2.3a (a vascular-specific high-affinity nitrate transporter) had reduced (by two-thirds) expression levels. At 60 min of nitrate treatment, lower expression levels were also observed for three additional NRT2 genes (OsNRT2.1/2.2/2.4). Conversely, in the overexpression lines, four NRT2 genes had much higher expression profiles at all time points tested. As previously reported, OsNRT2.3a functions in nitrate translocation, indicating the possible interaction between OsMADS57 and OsNRT2.3a. Yeast one-hybrid and transient expression assays demonstrated that OsMADS57 binds to the CArG motif (CATTTTATAG) within the OsNRT2.3a promoter. Moreover, seminal root elongation was inhibited in osmads57 mutants, which may be associated with higher auxin levels in and auxin polar transport to root tips of mutant plants. Taken together, these results suggest that OsMADS57 has a role in regulating nitrate translocation from root to shoot via OsNRT2.3a.

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