文献类型： 外文期刊
作者： Wang, Cong-Cong 1 ; Ke, Lei 1 ; Cao, Liang-Jing 2 ; Yao, Yuan 2 ; Geng, Meng-Ting 1 ; Wang, Ying 1 ; Xiao, Yu 1 ; Huang, 1 ;
作者机构： 1.Hainan Univ, Inst Trop Agr & Forestry, Hainan Key Lab Sustainable Utilisat Trop Bioresou, Haikou 570228, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China
关键词： MeMinE gene; plastid division; protoplast isolation; subcellular localization; prokaryotic expression
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
ISSN： 0916-8451
年卷期： 2019 年 83 卷 1 期
页码：
收录情况： SCI
摘要： The MinE protein plays an important role in plastid division. In this study, the MinE gene was isolated from the cassava (Manihot esculenta Crantz) genome. We isolated high quality and quantity protoplasts and succeed in performing the transient expression of the GFP-fused Manihot esculenta MinE (MeMinE) protein in cassava mesophyll protoplasts. The transient expression of MeMinE-GFP in cassava protoplasts showed that the MeMinE protein was located in the chloroplast. Due to the abnormal division of chloroplasts, overexpression of MeMinE proteins in cassava mesophyll protoplasts could result in fewer and smaller chloroplasts. Overexpression of MeMinE proteins also showed abnormal cell division characteristics and minicell occurrence in Escherichia coli caused by aberrant septation events in the cell poles.
- 相关文献
作者其他论文 更多>>
-
Climate-shaped vegetation dominated the spatial pattern of the Bowen ratio over terrestrial ecosystems in China
作者：Sun, Mingyu;Han, Lang;Yao, Yuan;Wang, Jilong;Yu, Guirui;Chen, Zhi;Hao, Tianxiang;Yang, Meng;Zhang, Weikang;Liu, Zhaogang;Ma, Lexin;Dou, Xiaojun;Luo, Wenxing;Lin, Yong;He, Yongtao;Shi, Peili;Wang, Huimin;Zhang, Yangjian;Zhao, Fenghua;Zhu, Zhilin;Yu, Guirui;Chen, Zhi;Liu, Zhaogang;Ma, Lexin;Dou, Xiaojun;Luo, Wenxing;Lin, Yong;He, Yongtao;Shi, Peili;Zhang, Yangjian;Chen, Zhi;Zhu, Xianjin;Han, Lang;Chen, Shiping;Deng, Zhengmiao;Duh, Hu;Wei, Wenxue;Dong, Gang;Gao, Yanhong;Ji, Xibin;Li, Yuqiang;Tan, Junlei;Gu, Fengxue;Hao, Xiangxiang;Hao, Yanbin;Hen, Qihua;He, Jinsheng;He, Jinsheng;He, Jinsheng;He, Jinsheng;Chen, Shiping;Jiang, Zhengde;Wu, Jiabing;Zhu, Jiaojun;Li, Xinhu;Li, Yingnian;Zhao, Liang;Li, Yuzhe;Zhang, Fawei;Liu, Shaomin;Luo, Weijun;Mo, Xingguo;Zhang, Yiping;Sha, Liqing;Song, Qinghai;Zhang, Yiping;Sun, Dan;Yan, Junhua;Tan, Junlei;Tang, Yakun;Wang, Fei;Wang, Jianlin;Wu, Zhixiang;Xin, Xiaoping;Xin, Xiaoping;Zhang, Yucui;Zhou, Li;Zhang, Weikang;Chen, Zhi
关键词：Bowen ratio; Heat flux; Sensible heat; Eddy covariance; Spatial patterns; Energy partitioning
-
Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava
作者：Lu, Xiao-Hua;Zhen, Xing-Hou;Pan, Mu;Hu, Xin-Wen;Wang, Ya-Jie;Yu, Hui;Li, Rui-Mei;Liu, Jiao;Yao, Yuan;Guo, Jian-Chun;Fu, Dong-Qing;Luo, Hai-Yan
关键词：cassava; starch synthase; MeSSIII-1; promoter; hormones; starch synthesis
-
Functional Analysis of the HbREF1 Promoter from Hevea brasiliensis and Its Response to Phytohormones
作者：Chen, Lin-Tao;Tang, Yan-Qiong;Peng, Shi-Qing;Chen, Lin-Tao;Guo, Dong;Zhu, Jia-Hong;Wang, Ying;Li, Hui-Liang;Peng, Shi-Qing;Guo, Dong;Zhu, Jia-Hong;Wang, Ying;Li, Hui-Liang;Peng, Shi-Qing;An, Feng
关键词：Hevea brasiliensis; rubber elongation factor; promoter; transcriptional regulation; phytohormone
-
TIP aquaporins in Cyperus esculentus: genome-wide identification, expression profiles, subcellular localizations, and interaction patterns
作者：Zou, Zhi;Zheng, Yujiao;Chang, Lili;Zou, Liangping;Zhao, Yongguo;Zhang, Li;Zhao, Yongguo;Min, Yi
关键词：Tigernut; Tonoplast intrinsic protein; Synteny analysis; Expression divergence; Subcellular localization; Protein interaction
-
Reprogramming of DNA methylation and changes of gene expression in grafted Hevea brasiliensis
作者：Li, Hui-Liang;Wang, Ying;Guo, Dong;Zhu, Jia-Hong;Wang, Yu;Dai, Hao-Fu;Peng, Shi-Qing;Li, Hui-Liang;Wang, Ying;Guo, Dong;Zhu, Jia-Hong;Wang, Yu;Dai, Hao-Fu;Peng, Shi-Qing;Li, Hui-Liang;Guo, Dong;Zhu, Jia-Hong;Wang, Yu;Li, Hui-Liang;Guo, Dong;Zhu, Jia-Hong;Wang, Yu;Li, Hui-Liang;Wang, Yu
关键词：Hevea brasiliensis; grafting; whole genome bisulfite sequencing; DNA methylation; gene expression
-
Promoter of Cassava MeAHL31 Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic Arabidopsis
作者：Wang, Xiao-Tong;Tang, Xiang-Ning;Zhang, Ya-Wen;Guo, Yu-Qiang;Yao, Yuan;Li, Rui-Mei;Wang, Ya-Jie;Liu, Jiao;Guo, Jian-Chun
关键词：cassava; AT-hook protein; promoter; GUS; transgenic Arabidopsis; abiotic stresses; hormone signals; MeAHL31
-
Transcriptomic study of the role of MeFtsZ2-1 in pigment accumulation in cassava leaves
作者：Zang, Yuwei;Wu, Kunlin;Liu, Liangwang;Ran, Fangfang;Wang, Changyi;Wu, Shuwen;Wang, Dayong;Min, Yi;Wang, Dayong;Wang, Dayong;Guo, Jianchun
关键词：MeFtsZ2-1; Cassava; Physiology; Transcriptome; Pigment accumulation
