文献类型： 外文期刊
作者： Wang, Cong-Cong 1 ; Ke, Lei 1 ; Cao, Liang-Jing 2 ; Yao, Yuan 2 ; Geng, Meng-Ting 1 ; Wang, Ying 1 ; Xiao, Yu 1 ; Huang, 1 ;
作者机构： 1.Hainan Univ, Inst Trop Agr & Forestry, Hainan Key Lab Sustainable Utilisat Trop Bioresou, Haikou 570228, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China
关键词： MeMinE gene; plastid division; protoplast isolation; subcellular localization; prokaryotic expression
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
ISSN： 0916-8451
年卷期： 2019 年 83 卷 1 期
页码：
收录情况： SCI
摘要： The MinE protein plays an important role in plastid division. In this study, the MinE gene was isolated from the cassava (Manihot esculenta Crantz) genome. We isolated high quality and quantity protoplasts and succeed in performing the transient expression of the GFP-fused Manihot esculenta MinE (MeMinE) protein in cassava mesophyll protoplasts. The transient expression of MeMinE-GFP in cassava protoplasts showed that the MeMinE protein was located in the chloroplast. Due to the abnormal division of chloroplasts, overexpression of MeMinE proteins in cassava mesophyll protoplasts could result in fewer and smaller chloroplasts. Overexpression of MeMinE proteins also showed abnormal cell division characteristics and minicell occurrence in Escherichia coli caused by aberrant septation events in the cell poles.
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