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ZmHsf05, a new heat shock transcription factor from Zea mays L. improves thermotolerance in Arabidopsis thaliana and rescues thermotolerance defects of the athsfa2 mutant

文献类型： 外文期刊

作者： Li, Guo-liang 1 ; Zhang, Hua-ning 1 ; Shao, Hongbo 5 ; Wang, Gui-yan 2 ; Zhang, Yuan-yuan 1 ; Zhang, Yu-jie 1 ; Zhao, 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Plant Genet Engn Ctr Hebei Prov, Inst Genet & Physiol, Shijiazhuang 050051, Hebei, Peoples R China

2.Hebei Agr Univ, Fac Agron, Baoding 071001, Peoples R China

3.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Hebei, Peoples R China

4.Hebei North Univ, Coll Agr & Forestry Sci & Technol, Zhangjiakou 075000, Peoples R China

5.Jiangsu Acad Agr Sci, Salt Soil Agr Ctr, Key Lab Agr Environm Lower Reaches Yangtze River, Inst Agr Resources & Environm, Nanjing 210014, Jiangsu, Peoples R China

6.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao 266000, Shandong, Peoples R China

7.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224002, Peoples R China

关键词： ZmHsf05; Thermotolerance; Heat shock transcription factor; Zea mays L.

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2019 年 283 卷

页码：

收录情况： SCI

摘要： High temperature directly affects the yield and quality of crops. Plant Hsfs play vital roles in plant response to heat shock. In the present study, ZmHsf05 was isolated from maize (Zea mays L.) using homologous cloning methods. The sequencing analysis demonstrated that CDS of ZmHsf05 was 1080 bp length and encoded a protein containing 359 amino acids. The putative amino acid sequence of ZmHsf05 contained typical Hsf domains, such as DBD, OD, NLS and AHA motif. Subcellular localization assays displayed that the ZmHsf05 is localized to the nucleus. ZmHsf05 was expressed in many maize tissues and its expression level was increased by heat stress treatment. ZmHsf05 rescued the reduced thermotolerance of the athsfa2 mutant in Arabidopsis seedlings. Arabidopsis seedlings of ZmHsf05-overexpressing increased both the basal and acquired thermotolerances. After heat stress, the ZmHsf05-overexpressing lines showed enhanced survival rate and chlorophyll content compared with WT seedlings. The expression of Hsps was up-regulated in the ZmHsf05-overexpressing Arabidopsis lines after heat stress treatment. These results suggested that ZmHsf05 plays an important role in both basal and acquired thermotolerance in plants.

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