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Detection and genetic characteristics of porcine circovirus 3 based on oral fluids from asymptomatic pigs in central China

文献类型： 外文期刊

作者： Guo, Zhenhua 1 ; Li, Xiang 4 ; Deng, Ruiguang 1 ; Zhang, Gaiping 1 ;

作者机构： 1.Henan Acad Agr Sci, Minist Agr, Henan Prov Key Lab Anim Immunol, Key Lab Anim Immunol, Zhengzhou, Henan, Peoples R China

2.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China

3.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

4.Yunnan Agr Univ, Dept Vet Med, Kunming, Yunnan, Peoples R China

关键词： PCV3; Epidemiology; Oral fluids; Phylogenetic analysis

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2019 年 15 卷

页码：

收录情况： SCI

摘要： BackgroundPorcine circovirus 3 (PCV3) is an emerging etiological agent to the swine industry. However, its circulating status and genetic characteristics were still unclear in Henan, central China. Here, 318 porcine oral fluid specimens were collected from asymptomatic pigs in five farms and tested by PCR .ResultsThe results showed that the positive rate of PCV3 was 12.3% (39/318) for the total samples, and 15.06% (25/166) in the stall-based samples, 9.21% (14/152) in the pen-based samples. Of the PCV3-positive samples, 41.0% were also positive for porcine circovirus 2 (PCV2). Nucleotide sequence comparison indicated that the 10 complete genomes and 34 capsid (cap) genes in this study shared 98.7-99.9% and 98-100% pairwise identities to each other, respectively. According to phylogenetic analysis and sequence alignment of cap gene, all the isolated sequences were clustered into 3 clades, including subgroup 1 (21/39, 61.8%), subgroup 2 (5/39, 14.7%) and subgroup 3 (8/39, 23.5%). Similar to previous reports, four amino acids (V24A, K27R, S77T and I150L) in cap protein were identified as a conserved subgroup specific molecular marker.ConclusionOur research provided new insights into the epidemiology surveillance and genetic characteristics of PCV3 in China.

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