Vitamin E stimulates the expression of gonadotropin hormones in primary pituitary cells of turbot (Scophthalmus maximus)
文献类型： 外文期刊
作者： Huang, Bin 1 ; Wang, Na 1 ; Wang, Lin 1 ; Jia, Yudong 1 ; Liu, Bin 1 ; Gao, Xiaoqiang 1 ; Liu, Baoliang 1 ; Wang, Weifang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
关键词： Nutrition and reproduction; Primary pituitary cell; Vitamin E; Gonadotropin hormones; Scophthalmus maximus
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2019 年 509 卷
页码：
收录情况： SCI
摘要： The relationship between vitamin E and gonadotropin hormones (FSH: follicle-stimulating hormone, LH: luteinizing hormone) in turbot was conducted by providing vitamin E through primary pituitary cells in vitro. Pituitaries were sampled aseptically and digested with 0.25% trypsin-EDTA for about 15 min at room temperature, and then were placed into the L-15 Medium, containing 5% fetal bovine serum, 100 U ml(-1) penicillin and 100 mu g ml(-1) streptomycin. After adding vitamin E (DL-alpha-tocopherol acetate from Sigma: 0, 9, 18, 36, 72 and 144 mu mol L-1) into the cells for one day, the cells were separated and collected for the mRNA analysis. Results showed that the mRNA expression of FSH-beta (fsh-b) were significantly increased when they were treated with vitamin E, and the significant increasing trends were dose-dependent with vitamin E concentrations; while the LH-beta (1h-b) mRNA expression were significantly increased with vitamin E from 0, 9 to 18 mu mol L-1 and not significantly increased any more. These data demonstrated that Vitamin E stimulates the expression of gonadotropin hormones in the pituitary of turbot in vitro. It also provides an alternative way to explore the mechanism of nutrient acting on the fish reproduction with this convenient cellular pituitary model.
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