Molecular characterization of G-type lysozyme from juvenile golden pompano Trachinotus ovatus and the regulation of its activity and expression by dietary sodium butyrate levels
文献类型： 外文期刊
作者： Zhou, Chuanpeng 1 ; Lin, Heizhao 2 ; Huang, Zhong 2 ; Wang, Jun 1 ; Wang, Yun 1 ; Yu, Wei 2 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr, Guangzhou 510300, Guangdong, Peoples R China
2.Chinese Acad Fishery Sci, Shenzhen Base, South China Sea Fisheries Res Inst, Shenzhen 518121, Peoples R China
关键词： Trachinotus ovatus; Sodium butyrate; G-type lysozyme; Enzyme activity; Gene expression; Ammonia stress
期刊名称：AQUACULTURE REPORTS （ 影响因子：3.216； 五年影响因子：3.812 ）
ISSN： 2352-5134
年卷期： 2019 年 14 卷
页码：
收录情况： SCI
摘要： The G-type lysozyme gene, non-specific immune molecule, was cloned from intestine of golden pompano (Trachinotus ovatus) by degenerate oligonucleotide primed PCR, including a complete open reading frame (ORF) of 804 bp. Homological protein analysis showed that the To-lysG in golden pompano shared a high degree of sequence identity with other known lysG sequences. In addition, tissue distribution analysis showed that To-lysG was expressed in nine tissues and had relatively high expression in spleen, head kidney and intestine. Then triplicate groups of golden pompano (18 cages) were fed twice daily to satiation with 0.0 (control), 0.25, 0.5, 1.0, 2.0 or 4.0 g kg(-1) sodium butyrate (SB) diets for 8 weeks, respectively. Compared to the control group, 2.0 and 4.0 g kg(-1) SB groups had higher lysozyme activities and the expression levels of To-lysG gene. After ammonia challenge, fish fed 1.0 g kg(-1) SB group showed the highest survival rate (P < 0.05). Overall, these results suggest that the intestinal lysozyme activity and mRNA expression of T. ovatus were up-regulated significantly as dietary SB levels increased. These results indicated that To-lysG plays a crucial role in the regulation of intestinal immune function of dietary graded levels of SB in T. ovatus.
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