您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line

文献类型： 外文期刊

作者： Ma, Hong 1 ; Fu, Bo 1 ; Zhang, Xu 1 ; Wang, Liang 1 ; Li, Zhongqiu 1 ; Liu, Di 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Harbin, Heilongjiang, Peoples R China

关键词： HSPC117; tissue expression; subcellular localization

期刊名称：TECHNOLOGY AND HEALTH CARE （ 影响因子：1.285； 五年影响因子：1.116 ）

ISSN： 0928-7329

年卷期： 2019 年 27 卷

页码：

收录情况： SCI

摘要： BACKGROUND: The human hematopoietic stem/progenitor cell 117 (HSPC117) protein is involved in many important biological processes. OBJECTIVE: This study was designed to identify the level of HSPC117 mRNA expression in 10 min pig tissue samples and HSPC117 subcellular localization in the PK15 cell line. METHODS: In this study, 10 tissue samples of min pigs were collected, and EGFP-HSPC117 vectors were constructed to express EGFP-HSPC117 fusion proteins in PK15 cells. RESULTS: HSPC117 mRNA was expressed in all of the tissue samples, although the levels of expression in fat and lung tissues were significantly lower than in other tissues (P < 0.01). After generating and detecting the EGFP-HSPC117 fusion protein, fluorescence was found to be distributed throughout the cytoplasm and nucleus during interphase; however, the fluorescence was concentrated in the nuclear area in mitotic cells. CONCLUSIONS: These results indicate that the HSPC117 gene is expressed in many min pig tissues. The HSPC117 protein was distributed throughout the cells during interphase, but was concentrated in the nuclear area in mitotic cells.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台