文献类型： 外文期刊
作者： Han, Kaikai 1 ; Zhao, Dongmin 1 ; Liu, Yuzhuo 1 ; Liu, Qingtao 1 ; Huang, Xinmei 1 ; Yang, Jing 1 ; Zhang, Lijiao 1 ; Li, 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Co Innovat Ctr Prevent & Control Importan, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod,Minist Agr,Inst Vet, Yangzhou 225009, Jiangsu, Peoples R China
关键词： Duck tembusu virus; Ubiquitin-proteasome system; Viral replication
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2019 年 132 卷
页码：
收录情况： SCI
摘要： Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. The cellular factors required for DTMUV replication have been poorly studied. The ubiquitin-proteasome system (UPS), the major intracellular proteolytic pathway, mediates diverse cellular processes, including endocytosis and signal transduction, which may be involved in the entry of virus. In the present study, we explored the interplay between DTMUV replication and the UPS in BHK-21 cells and found that treatment with proteasome inhibitor (MG132 and lactacystin) significantly decreased the DTMUV progency at the early infection stage. We further revealed that inhibition of the UPS mainly occurs on the level of viral protein expression and RNA transcription. In addition, using specific siRNAs targeting ubiquitin reduces the production of viral progeny. In the presence of MG132 the staining for the envelope protein of DTMUV was dramatically reduced in comparison with the untreated control cells. Overall, our observations reveal an important role of the UPS in multiple steps of the DTMUV infection cycle and identify the UPS as a potential drug target to modulate the impact of DTMUV infection.
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