文献类型： 外文期刊
作者： Chen, Xiaolan 1 ; Ouyang, Hongjia 1 ; Chen, Biao 1 ; Li, Guihuan 1 ; Wang, Zhijun 1 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
2.Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
3.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
关键词： miR-223; EIF5A2; SNPs; Myoblast; Proliferation; Differentiation
期刊名称：LIVESTOCK SCIENCE （ 影响因子：1.943； 五年影响因子：2.252 ）
ISSN： 1871-1413
年卷期： 2019 年 225 卷
页码：
收录情况： SCI
摘要： Eukaryotic translation initiation factor 5A2 (EIF5A2) is an essential protein closely related to cellular polyamine homeostasis. It is the only cellular protein that contains the unusual amino acid hypusine (N-epsilon-(4-amino-2-hydroxybutyl) lysine] which is necessary for the biochemical activity of EIF5A2 and cellular proliferation. In this study, we aim to characterize chicken EIF5A2 and explore its potential effects on skeletal muscle cell proliferation and differentiation. Here, we found that chicken EIF5A2 has four variant transcripts. The single nucleotide polymorphisms (SNPs) in the 5'-flanking region of EIF5A2 were significantly associated with the chicken growth and fatness traits. Expression pattern of EIFSA2 in skeletal muscle revealed that it was highly expressed at an early embryonic age and then decreased sharply after d 15 of the embryonic age. The luciferase reporter assays confirmed that EIF5A2 was directly targeted by miRNA-223. EIF5A2 was negatively regulated by miR-223 in myoblast. Both 5-Ethynyl-2'-deoxyuridine staining assays and flow cytometry analyses of the cell cycle showed that down-regulation of EIF5A2 could markedly inhibit myoblast proliferation. We found that down regulation of EIF5A2 could promote myoblast differentiation through promoting the expression of Myogenin (MYOG). MYOG is a muscle-specific transcription factor involved in myogenesis. Altogether, the results indicated that EIF5A2 is an important candidate gene in chicken growth, and down-regulation of EIF5A2 could suppress myoblast proliferation and promote myoblast differentiation by targeting miR-223.
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