文献类型： 外文期刊

作者： Wu, J. ¹ ; Chen, Q. ³ ; Xin, T. ¹ ; Sun, Y. ⁵ ; Jia, H. ¹ ; Hou, S. H. ¹ ; Guo, X. Y. ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Dept Vet Med, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China

2.Univ Liege, Gembloux Agrobio Tech, Lab Mol & Cellular Biol, Passage Deportees 2, B-5030 Gembloux, Belgium

3.Beijing Agr Univ, Coll Biol Sci & Engn, 7 Beinong Rd, Beijing 102206, Peoples R China

4.Minist Agr, Key Lab Urban Agr North China, 7 Beinong Rd, Beijing 102206, Peoples R China

5.China Anim Husb Ind Co Ltd, 6 Huansan Rd, Beijing 100070, Peoples R China

关键词： adjuvant; CpG DNA; Echinococcus granulosus; TBK1; type I interferons

期刊名称：POLISH JOURNAL OF VETERINARY SCIENCES （ 影响因子：0.821； 五年影响因子：1.005 ）

ISSN： 1505-1773

年卷期： 2019 年 22 卷 2 期

页码：

收录情况： SCI

摘要： Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-beta, and pro-inflammatory cytokines TNF-alpha and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adjuvanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response.