Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure
文献类型： 外文期刊
作者： Shen, Qingshan 1 ; Zhang, Chunhui 1 ; Jia, Wei 1 ; Qin, Xiaojie 1 ; Cui, Zhenkun 2 ; Mo, Haizhen 2 ; Richel, Aurore 3 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Food Sci & Technol, Minist Agr & Rural Affairs, Key Lab Agroprod Proc, Beijing 100193, Peoples R China
2.Henan Inst Sci & Technol, Dept Food Sci, Xinxiang 453003, Henan, Peoples R China
3.Univ Liege Gembloux Agrobio Tech, Lab Biomass & Green Technol, Passage Deportes 2, B-5030 Gembloux, Belgium
关键词： Hot-pressure; Co-production; Liquefaction; Chondroitin sulfate; Peptides
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）
ISSN： 0144-8617
年卷期： 2019 年 222 卷
页码：
收录情况： SCI
摘要： Co-production of chondroitin sulfate (CS) and peptides was realized from the liquid fraction of chicken sternal cartilage subjected to hot-pressure (HP) by membrane combination separation technology. Cartilage was liquefied via the HP treatment at 110 degrees C (0.07 MPa) and 120 degrees C (0.1 MPa) for 0.5-2.5 h, respectively. The optimized co-production procedure was as follows:enzymolysis temperature, 61.2 degrees C; the enzyme ratio of trypsin and papain, 1.3:1 (W/W); enzymolysis time ratio, 2:2 (h/h), under which the highest yields of CS and peptides were 18.85% and 67.99%, and the recoveries were 93.63% and 92.69%. The average molecular weight of CS sample was 67.79 kDa. CS sample was confirmed using agarose-gel electrophoresis, and the structure was analyzed by Fourier transform infrared spectroscopy, chromatography and nuclear magnetic resonance. Taken together, HP can be as a pretreatment method to liquefy cartilage for the industrial co-production of CS and peptides with eco-friendly.
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