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Comparative genomic analysis unravels the transmission pattern and intra-species divergence of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strains

文献类型： 外文期刊

作者： Yang, Qian 1 ; Dong, Xuan 1 ; Xie, Guosi 1 ; Fu, Songzhe 2 ; Zou, Peizhuo 1 ; Sun, Jing 1 ; Wang, Yi 2 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Lab Marine Fisheries Sci & Food Prod Proc,Qingdao, Yellow Sea Fisheries Res Inst,Qingdao Natl Lab Ma, Key Lab Maricultural Organism Dis Control,Minist, Qingdao, Shandong, Peoples R China

2.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian, Peoples R China

关键词： AHPND; Vibrio parahaemolyticus; SNPs; Whole-genome sequencing; Genomic plasticity

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：3.291； 五年影响因子：3.257 ）

ISSN： 1617-4615

年卷期： 2019 年 294 卷 4 期

页码：

收录情况： SCI

摘要： Acute hepatopancreatic necrosis disease (AHPND) is a recently discovered shrimp disease that has become a severe threat to global shrimp-farming industry. The causing agents of AHPND were identified as Vibrio parahaemolyticus and other vibrios harboring a plasmid encoding binary toxins PirA(vp)/PirB(vp). However, the epidemiological involvement of environmental vibrios in AHPND is poorly understood. In this study, with an aim to reveal the possible transmission route of AHPND-causing V. parahaemolyticus, we sequenced and analyzed the genomes of four pairs of V. parahaemolyticus strains from four representative regions of shrimp farming in China, each including one strain isolated from diseased shrimp during an AHPND outbreak and one strain isolated from sediment before AHPND outbreaks. Our results showed that all the four shrimp-isolated and three of the sediment-isolated strains encode and secret PirA(vp)/PirB(vp) toxins and, therefore, are AHPND-causing strains. In silico multilocus sequence typing and high-resolution phylogenomic analysis based on single-nucleotide polymorphisms, as well as comparison of genomic loci in association with prophages and capsular polysaccharides (CPSs) consistently pointed to a close genetic relationship between the shrimp- and sediment-isolated strains obtained from the same region. In addition, our analyses revealed that the sequences associated with prophages, CPSs, and type VI secretion system-1 are highly divergent among strains from different regions, implying that these genes may play vital roles in environmental adaptation for AHPND-causing V. parahaemolyticus and thereby be potential targets for AHPND control. Summing up, this study provides the first direct evidence regarding the transmission route of AHPND-causing V. parahaemolyticus and underscores that V. parahaemolyticus in shrimp are most likely originated from local environment. The importance of environmental disinfection measures in shrimp farming was highlighted.

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