A positive-sense single-stranded RNA virus acquired a negative-sense open reading frame through recombination
文献类型： 外文期刊
作者： Dong, Xuan 1 ; Zhang, Fan 1 ; Wang, Yiting 1 ; Li, Cixiu 4 ; Li, Xuan 1 ; Zhou, Chengyan 1 ; Wang, Guohao 1 ; Tian, Zhongshuai 5 ; Gao, Jing 6 ; Zhou, Hu 6 ; Sui, Liying 7 ; Nauwynck, Hans 8 ; Sorgeloos, Patrick 9 ; Holmes, Edward C. 10 ; Huang, Jie 1 ; Shi, Weifeng 5 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao, Peoples R China
4.Shandong First Med Univ & Shandong Acad Med Sci, Key Lab Emerging Infect Dis Univ Shandong, Jinan, Peoples R China
5.Shanghai Jiao Tong Univ, Shanghai Inst Virol, Sch Med, Shanghai, Peoples R China
6.Chinese Acad Sci, Dept Analyt Chem, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai, Peoples R China
7.Tianjin Univ Sci & Technol, Asian Reg Artemia Reference Ctr, Tianjin, Peoples R China
8.Univ Ghent, Fac Vet Med, Lab Virol, Ghent, Belgium
9.Univ Ghent, Fac Biosci Engn, Lab Aquaculture & Artemia Reference Ctr, Ghent, Belgium
10.Univ Sydney, Sch Med Sci, Sydney, NSW, Australia
11.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai, Peoples R China
期刊名称：PLOS PATHOGENS （ 影响因子：4.9； 五年影响因子：5.4 ）
ISSN： 1553-7366
年卷期： 2025 年 21 卷 4 期
页码：
收录情况： SCI
摘要： Although positive- and negative-sense single-stranded RNA viruses are ubiquitous in nature, there is currently no evidence of recombination or reassortment between viruses with these two major forms of genome organization. Here, we describe the discovery of brine shrimp virga-like virus 1 (BSVV1), a novel positive-sense single-stranded RNA virus with a recombinant genome structure derived from two viral phyla with differing genome organizations. The genome of BSVV1 comprises three open reading frames (ORFs). ORF1 resembles the RNA-dependent RNA polymerase of Ips virga-like virus 1 (a positive-sense RNA virus), while ORF2, transcribed in the positive orientation, is related to the glycoprotein of Hubei bunya-like virus 10 and other negative-sense RNA viruses. The predicted ORF3 was unique to BSVV1 without known homologs identified. The presence of the three protein products was verified by mass spectrometry. Notably, our analysis also revealed that BSVV1 is geographically widespread and found in brine shrimp from at least eight countries on four continents. In addition, BSVV1 was successfully cultured and proliferated to high viral loads during brine shrimp development. In sum, we provide compelling evidence of an ancient recombination event between negative- and positive-sense single-stranded RNA viruses, enriching our understanding of the evolution of genome structures in RNA viruses.
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