文献类型： 外文期刊
作者： Xie, Guosi 1 ; Wang, Hailiang 2 ; Zhu, Mengting 3 ; Bi, Jingnan 3 ; Yang, Liuxin 3 ; Wan, Xiaoyuan 1 ; Li, Meng 2 ; Xie, Enpei 3 ; Shi, Chengyin 1 ; Yang, Bing 1 ; Zhang, Qingli 1 ; Li, Caiwen 2 ; Huang, Jie 1 ;
作者机构： 1.Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst YSFRI, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao, Shandong, Peoples R China
2.Laoshan Lab, Qingdao, Shandong, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao, Shandong, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
关键词：
detection;
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.2； 五年影响因子：2.2 ）
ISSN： 0140-7775
年卷期： 2025 年 48 卷 5 期
页码：
收录情况： SCI
摘要： Hematodinium perezi, a pathogenic dinoflagellate, is one of major epidemiological agents that lead to severe losses of cultured marine crustaceans in China. This study aimed to develop a novel, sensitive and specific detection method qualified for early surveillance and control of the disease caused by H. perezi. The present study established a TaqMan-MGB probe quantitative PCR (qPCR) method, targeting the first internal transcribed spacer 1 (ITS 1) region of H. perezi by optimising reaction components. A high correlation coefficient (R2 = 0.9996) was obtained in a standard curve with a 103.4% efficiency. No amplification was observed for the host's genome and pathogens other than H. perezi in the TaqMan-MGB probe qPCR assays, showing high specificity to H. perezi. When using the plasmid standard DNA as templates, the detection limit of the qPCR method was determined to be 5.66 copies/reaction and 10 times more sensitive than the conventional PCR. The TaqMan-MGB probe qPCR assays exhibited high repeatability, and the intra- and inter-assay coefficients of variation (CV) ranged from 0.11% to 2.25% over a wide dynamic range of detection from 5.66 x 100 to 5.66 x 109 copies of targeting gene. The application was also validated on clinical samples, including those with low infection with H. perezi. This novel one-step TaqMan-MGB probe qPCR provides an option for surveillance and epidemiological investigations of H. perezi infection, with an advantage at the early infection stage.
- 相关文献
作者其他论文 更多>>
-
The 2023 National Proficiency Test for molecular detection of shrimp infectious myonecrosis virus (IMNV) in China
作者：Li, Chen;Yang, Bing;Wang, Meifeng;Wan, Xiaoyuan;Li, Wenqiang;Song, Xiaoling;Liu, Li;Zhang, Qingli;Li, Chen;Yang, Bing;Wan, Xiaoyuan;Zhang, Qingli;Yu, Weizhong;Li, Qing;Wang, Meifeng;Li, Wenqiang
关键词：Proficiency test (PT); Infectious myonecrosis virus (IMNV); Z-score
-
Evaluation of the flinders technology associates cards for storage and molecular detection of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) DNA
作者：Lian, Xin-Yu;Wang, Xiu-Hua;Li, Chen;Zhang, Qing-Li;Lu, Ruo-Xuan;Gou, Zi-Yue;Xu, Hua;Wang, Mei-Feng;Yang, Bing;Wang, Xiu-Hua;Zhang, Qing-Li;Yang, Bing;Lian, Xin-Yu;Wang, Xiu-Hua;Li, Chen;Zhang, Qing-Li;Lu, Ruo-Xuan;Gou, Zi-Yue;Xu, Hua;Wang, Mei-Feng;Yang, Bing;Jia, Peng
关键词：IHHNV; Flinders technology associates cards; Real-time PCR
-
Horizontal and vertical transmission of covert mortality nodavirus in the marine fish
作者：Xu, Tingting;Xia, Jitao;Wang, Chong;Li, Yingxia;Zhang, Qingli;Xu, Tingting;Xia, Jitao;Wang, Chong;Li, Yingxia;Zhang, Qingli;Li, Jun
关键词：Covert mortality nodavirus (CMNV); Oryzias melastigma; Model organism; Vertical transmission; Horizontal transmission
-
Vibrio plasmids harboring vhv gene associated with shrimp translucent post-larvae disease: Coexistence of two types of T4SS and multiple transposons
作者：Tan, Peilong;Yang, Mingshu;Zhang, Yuying;Zhang, Qingli;Liang, Xiaosha
关键词：Translucent post-larvae disease; Vhv -positive plasmids; Type IV Secretion System; Transposons; Shrimp aquaculture
-
RNA virus diversity highlights the potential biosecurity threat posed by Antarctic krill
作者：Xu, Tingting;Zhao, Xianyong;Zhu, Jiancheng;Wang, Wei;Wang, Xinliang;Wang, Chong;Fan, Gangzhou;Zhang, Jichang;Zhao, Wenxiu;Zhang, Qingli;Xu, Tingting;Zhao, Xianyong;Zhu, Jiancheng;Wang, Wei;Wang, Xinliang;Wang, Chong;Fan, Gangzhou;Zhang, Jichang;Zhao, Wenxiu;Zhang, Qingli;Xu, Tingting;Zhao, Xianyong;Zhu, Jiancheng;Wang, Wei;Wang, Xinliang;Wang, Chong;Fan, Gangzhou;Zhang, Jichang;Zhao, Wenxiu;Zhang, Qingli;Loch, Thomas;Hao, Bin;Bondad-Reantaso, Melba G.;Alday-Sanz, Victoria
关键词：RNA virome; Antarctic krill
Euphausia superba ; Biosafety; Global aquaculture; Antarctic ecosystem -
Effects of multiscale environmental variables on the taxonomic and functional structures of riverine microeukaryotic plankton communities: eDNA metabarcoding and metatranscriptomic perspectives
作者：Luo, Rongxu;Wang, Shuping;Zou, Hongguang;Kong, Weijing;Li, Meng;Zhang, Yaqun;Mo, Lidong
关键词：Microeukaryotic plankton; River; eDNA; eRNA; Environmental variable
-
Penaeus chinensis nearing a critical threshold of susceptibility to Decapod iridescent virus 1 (DIV1)
作者：Guo, Xiao-Meng;Wang, Guo-Hao;Xing, Jing-Yi;Qiu, Liang;Huang, Jie;Qiu, Liang;Huang, Jie;Yao, Jia-Yun;Guo, Xiao-Meng;Wang, Guo-Hao;Huang, Jie;Chen, Xiao-Ling;Xing, Jing-Yi
关键词：Decapod iridescent virus 1; Susceptible species; Penaeus chinensis; Non-invasive challenge mimicking nature; transmission pathway
