您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Horizontal and vertical transmission of covert mortality nodavirus in the marine fish

文献类型： 外文期刊

作者： Xu, Tingting 1 ; Xia, Jitao 1 ; Wang, Chong 1 ; Li, Yingxia 1 ; Li, Jun 3 ; Zhang, Qingli 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Key Lab Maricultural Organism Dis Control,Minist A, Qingdao 266071, Peoples R China

2.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

3.Lake Super State Univ, Sch Sci & Med, Sault Ste Marie, MI 49783 USA

关键词： Covert mortality nodavirus (CMNV); Oryzias melastigma; Model organism; Vertical transmission; Horizontal transmission

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 607 卷

页码：

收录情况： SCI

摘要： Covert mortality nodavirus (CMNV) is a vital emerging virus in aquaculture issued by World Organization for Animal Health recently. It can infect teleost fish, but the mode of transmission remains unclear. Therefore, this study investigated the potential of vertical and horizontal transmission of CMNV in marine fish using the marine model organism of marine medaka Oryzias melastigma. First, the clinical symptoms, viral load and histopathological changes of marine medaka artificially infected by intraperitoneal injection infection were consistent with those of fish species naturally infected with CMNV, indicating that marine medaka is an ideal model organism for CMNV infection. Subsequently, RT-nPCR analysis demonstrated a 10.5 % CMNV positivity rate in cohabitation-challenged marine medaka, with viral loads ranging from 3.28 x 102 to 3.21 x 103 copies per gram of tissue. Histopathological analysis revealed severe lesions occurred in the brain, eye, kidney, and intestine of infected individuals, with positive hybridization signals of CMNV RNA probes were detected at necrotic sites in in situ hybridization (ISH) assay. These results provide strong evidence for the horizontal transmission of CMNV through waterborne in marine medaka. In addition, RT-nPCR and ISH analysis showed that ovarian tissue, testis tissue, fertilized eggs/embryos, and offspring fish from artificially infected parental marine medaka all tested positive for CMNV, demonstrating that vertical transmission can occur through either parent. The results of this study provide compelling evidence for both vertical and horizontal modes of transmission of CMNV in marine medaka.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台