Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination
文献类型： 外文期刊
作者： Xu, Chongxin 1 ; Han, Qi 1 ; Dong, Sa 1 ; Liu, Xiaoqin 3 ; Liu, Xianjin 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Nanjing, Jiangsu, Peoples R China
2.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou, Jiangsu, Peoples R China
3.Huaihua Vocat & Tech Coll, Dept Anim Sci & Technol, Huaihua, Peoples R China
关键词： Vancomycin; polyclonal antibodies; ELISA; IC-TRFIA; milk powder
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2019 年 30 卷 1 期
页码：
收录情况： SCI
摘要： Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive time-resolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong cross-reactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stability and repeatability, and its recoveries are 83.0-97.9%, also with its coefficient of variations (CVs) are 3.0-12.9% in intra-assay and inter-assay. The results showed that the established IC-TRFIA was promising for ultrasensitive determination of Van in food samples.
- 相关文献
作者其他论文 更多>>
-
Generation of anti-idiotypic antibodies mimicking Cry2Aa toxin from an immunized mouse phage display library as potential insecticidal agents against Plutella xylostella
作者：Lin, Manman;Liu, Yuan;Zhang, Xiao;Xu, Chongxin;Hu, Xiaodan;Zhu, Qing;Xie, Yajing;Chen, Wei;Liu, Xianjin;Lin, Manman;Hu, Xiaodan;Xie, Yajing;Lin, Johnson;Liu, Yuan;Meng, Meng;Jin, Jiafeng;Shen, Cheng
关键词：Anti-idiotypic antibody; Single-chain variable fragment; Cry2Aa toxin; Insecticidal protein; Plutella xylostella
-
Enhanced composite Co-MOF-derived sodium carboxymethyl cellulose visual films for real-time and in situ monitoring fresh-cut apple freshness
作者：Zhang, Zhepeng;Zhang, Yang;Wang, Chen;Zou, Xiaobo;Guo, Zhiming;Liu, Xianjin;El-Seedi, Hesham R.;Gomez, Paula L.;Alzamora, Stella M.
关键词：Metal-organic framework; Multifunctional nano-filler; Sodium carboxymethyl cellulose; Intelligent film; Freshness monitoring
-
Preparation of monoclonal antibody and establishment of indirect competitive chemiluminescence enzyme immunoassay for sodium pentachlorophenolate
作者：Dong, Sa;Shi, Qiuyun;Guan, Lingjun;Feng, Jianguo;Guan, Lingjun;Wang, Yulong;Liu, Pengyan;Zhang, Cunzheng
关键词：Sodium pentachlorophenate; Monoclonal antibody; ic-CLEIA; Residual detection; Animal tissues
-
Rational design and application of broad-spectrum antibodies for Bt Cry toxins determination
作者：Jin, Jiafeng;Chen, Wei;Xu, Chongxin;Xie, Yajing;Shen, Cheng;Meng, Meng;Zhu, Qin;Zhang, Xiao;Liu, Xianjin;Liu, Yuan;Jin, Jiafeng;Xu, Chongxin;Liu, Yuan;Jin, Jiafeng;Shen, Cheng;Pooe, Ofentse Jacob;Meng, Meng
关键词：Bt Cry toxins; Polyclonal antibodies; Monoclonal antibody; ELISA; Genetically modified crops
-
Evaluation of Temperature Uniformity in a Middle-Refrigerated Truck Loaded with Pig Carcasses
作者：Bai, Hongwu;Zhou, Guanghong;Bai, Hongwu;Liu, Xianjin
关键词：refrigerated truck; computational fluid dynamics; carcass traceability; temperature field
-
Transcriptome analysis provides new insight into the distribution and transport of selenium and its associated metals in selenium-rich rice
作者：Jiao, Linshu;Zhang, Liuquan;Zhang, Yongzhu;Wang, Ran;Lu, Baiyi;Liu, Xianjin;Zhang, Liuquan;Lu, Baiyi
关键词：Selenium; Heavy metal; Rice (Oryza sativa L; ); Transporter; Antioxidant
-
Screening and identification of vancomycin anti-idiotypic antibodies for against Staphylococcus aureus from a human phage display domain antibody library
作者：Xu, Chongxin;Yu, Mingzheng;Xie, Yajing;Zhong, Jianfeng;Chen, Wei;Lin, Manman;Hu, Xiaodan;Shen, Yan;Xu, Chongxin
关键词：Vancomycin; Staphylococcus aureus; Phage display antibody library; Anti-idiotypic antibody
