Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination
文献类型： 外文期刊
作者： Xu, Chongxin 1 ; Han, Qi 1 ; Dong, Sa 1 ; Liu, Xiaoqin 3 ; Liu, Xianjin 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Nanjing, Jiangsu, Peoples R China
2.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou, Jiangsu, Peoples R China
3.Huaihua Vocat & Tech Coll, Dept Anim Sci & Technol, Huaihua, Peoples R China
关键词： Vancomycin; polyclonal antibodies; ELISA; IC-TRFIA; milk powder
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2019 年 30 卷 1 期
页码：
收录情况： SCI
摘要： Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive time-resolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong cross-reactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stability and repeatability, and its recoveries are 83.0-97.9%, also with its coefficient of variations (CVs) are 3.0-12.9% in intra-assay and inter-assay. The results showed that the established IC-TRFIA was promising for ultrasensitive determination of Van in food samples.
- 相关文献
作者其他论文 更多>>
-
Combined detection of foodborne pathogens in irrigation water in Jiangsu, China
作者：Xie, Yajing;Zhang, Cunzheng;Liu, Xianjin;Xie, Yajing;Lu, Jiayao;Liu, Xianjin;Li, Bin;Fan, Bochao;Li, Dandi;Du, Xuefei;Xie, Yajing;Schmidt, Stefan
关键词：Irrigation water; Foodborne pathogens; Combined detection; Noroviruses; Strawberry production
-
A fast and efficient virtual screening and identification strategy for helix peptide binders based on finDr webserver: A case study of bovine serum albumin (BSA)
作者：Bu, Jiarui;Luo, Na;Liu, Yuan;Luo, Chuping;Zhang, Xiao;Bu, Jiarui;Luo, Na;Shen, Cheng;Xu, Chongxin;Zhu, Qing;Chen, Chengyu;Xie, Yajing;Liu, Xianjin;Liu, Yuan;Zhang, Xiao
关键词：Virtual screening; Affinity identification; Molecular simulations
-
An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking
作者：Lin, Manman;Han, Guangjie;Li, Chuanming;Huang, Lixin;Liu, Qin;Zhang, Nan;Xia, Yang;Lu, Yurong;Xu, Jian;Liu, Xianjin;Liu, Yuan;Lin, Johnson
关键词：Cry2Aa toxin; Anti-idiotypic antibody; Single-domain antibody; Epitopes; Mimotope; Molecular docking
-
Development of colorimetric immunochromatographic assay based on different morphologies of gold nanoparticles for the detection of triadimefon
作者：Dong, Sa;Zhang, Yu;Shi, Qiuyun;Zhu, Zixin;Feng, Jianguo;Wang, Yulong;Luo, Ruifeng
关键词：Triadimefon; Immunochromatographic strip; Gold nanoparticles; Gold nanoflowers; Gold nanorods; Monoclonal antibody
-
Minimalist nanobody architecture: A double-edged sword for immunological analysis
作者：Wang, Yulong;Liu, Pengyan;Zhang, Yaodong;Zhang, Cunzheng;Wang, Yulong;Luo, Ruifeng;Yang, Huijuan;Dong, Sa;Hua, Xiude;Hammock, Bruce D.
关键词：Nanobody-based immunoassay; Miniaturized structure; Small size; Bioconjugation chemistry
-
Effects of neuropeptide F signaling on feeding, growth and development of Plutella xylostella (L.) larvae
作者：Shen, Cheng;Wu, Jiayi;Huang, Ziyan;He, Meng;Chen, Wei;Ilyas, Naila;Zhang, Xiao;Chen, Chengyu;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Shen, Cheng;Huang, Ziyan;Liu, Yuan;Liu, Xianjin;Wu, Jiayi;He, Meng;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Wang, Yun
关键词：Plutella xylostella; Neuropeptide F signaling; Feeding regulation
-
Generation of anti-idiotypic antibodies mimicking Cry2Aa toxin from an immunized mouse phage display library as potential insecticidal agents against Plutella xylostella
作者：Lin, Manman;Liu, Yuan;Zhang, Xiao;Xu, Chongxin;Hu, Xiaodan;Zhu, Qing;Xie, Yajing;Chen, Wei;Liu, Xianjin;Lin, Manman;Hu, Xiaodan;Xie, Yajing;Lin, Johnson;Liu, Yuan;Meng, Meng;Jin, Jiafeng;Shen, Cheng
关键词：Anti-idiotypic antibody; Single-chain variable fragment; Cry2Aa toxin; Insecticidal protein; Plutella xylostella
