文献类型： 外文期刊

作者： Niu, Suzhen ¹ ; Song, Qinfei ¹ ; Koiwa, Hisashi ² ; Qiao, Dahe ³ ; Zhao, Degang ¹ ; Chen, Zhengwu ³ ; Liu, Xia ¹ ; Wen, X ¹ ;

作者机构： 1.Guizhou Univ, Key Lab Plant Resources Conservat & Germplasm Inn, Inst Agrobioengn, Minist Educ,Coll Tea Sci, Guiyang 550025, Guizhou, Peoples R China

2.Texas A&M Univ, Dept Hort Sci, Vegetable & Fruit Improvement Ctr, Mol & Environm Plant Sci Program, MS2133, College Stn, TX 77843 USA

3.Guizhou Acad Agr Sci, Inst Tea, Guiyang 550006, Guizhou, Peoples R China

4.Guizhou Univ, Inst Agrobioengn, Coll Life Sci, Huaxi Ave, Guiyang 550025, Guizhou, Peoples R China

5.Guizhou Univ, Minist Educ, Key Lab Plant Resources Conservat & Germplasm Inn, Xiahui Rd, Guiyang 550025, Guizhou, Peoples R China

关键词： Genotyping-by-sequencing; Population structure; Linkage disequilibrium; Genetic diversity; Tea plant; Origin center; Guizhou plateau

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2019 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundTo efficiently protect and exploit germplasm resources for marker development and breeding purposes, we must accurately depict the features of the tea populations. This study focuses on the Camellia sinensis (C. sinensis) population and aims to (i) identify single nucleotide polymorphisms (SNPs) on the genome level, (ii) investigate the genetic diversity and population structure, and (iii) characterize the linkage disequilibrium (LD) pattern to facilitate next genome-wide association mapping and marker-assisted selection.ResultsWe collected 415 tea accessions from the Origin Center and analyzed the genetic diversity, population structure and LD pattern using the genotyping-by-sequencing (GBS) approach. A total of 79,016 high-quality SNPs were identified; the polymorphism information content (PIC) and genetic diversity (GD) based on these SNPs showed a higher level of genetic diversity in cultivated type than in wild type. The 415 accessions were clustered into three groups by STRUCTURE software and confirmed using principal component analyses (PCA)-wild type, cultivated type, and admixed wild type. However, unweighted pair group method with arithmetic mean (UPGMA) trees indicated the accessions should be grouped into more clusters. Further analyses identified four groups, the Pure Wild Type, Admixed Wild Type, ancient landraces and modern landraces using STRUCTURE, and the results were confirmed by PCA and UPGMA tree method. A higher level of genetic diversity was detected in ancient landraces and Admixed Wild Type than that in the Pure Wild Type and modern landraces. The highest differentiation was between the Pure Wild Type and modern landraces. A relatively fast LD decay with a short range (kb) was observed, and the LD decays of four inferred populations were different.ConclusionsThis study is, to our knowledge, the first population genetic analysis of tea germplasm from the Origin Center, Guizhou Plateau, using GBS. The LD pattern, population structure and genetic differentiation of the tea population revealed by our study will benefit further genetic studies, germplasm protection, and breeding.