您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

QTL mapping and gene mining to identify genes on soybean (Glycine max) associated with NopB of Sinorhizobium fredii HH103

文献类型： 外文期刊

作者： Wang, Jieqi 1 ; Wang, Jinhui 1 ; Tian, Boyu 1 ; Li, Qingying 1 ; Zhu, Jingyi 1 ; Liu, Xueying 1 ; Ma, Chao 1 ; Li, Changy 1 ;

作者机构： 1.Northeast Agr Univ, Coll Sci, Chinese Minist Educ,Key Lab Soybean Biol, Key Lab Soybean Biol & Breeding,Genet Chinese Agr, Harbin, Heilongjiang, Peoples R China

2.Heilongjiang Acad Agr Sci, Harbin, Heilongjiang, Peoples R China

关键词： nodulation; NopB; QTL; rhizobium; soybean; symbiosis

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN： 0179-9541

年卷期：

页码：

收录情况： SCI

摘要： Soybean is a special crop that can utilize N-2 in the air via symbioses with Rhizobium spp. The formation of effective nodules is a complex process in which nodulation outer proteins (Nops) are determinants of establishment of a symbiotic relationship. We constructed a Sinorhizobium fredii HH103 omega nopB mutant. A nodulation test showed that the mutant had a negative effect on the Suinong14, ZYD00006, Dongnong594 and Charleston soybean lines. Recombinant inbred soybean lines were independently inoculated with the mutant and wild-type strains, and five and four quantitative trait loci (QTLs) were identified by analysing the nodule number (NN) and nodule dry weight (NDW), respectively. We chose one QTL that overlapped with other studies and a novel QTL identified in our study and selected six candidate genes for further analysis. The qRT-PCR analysis showed that only changes in Glyma.17G166200 expression depended on NopB. Further analysis showed that Glyma.17G166200 encoded a protein with a D-glucose-binding domain and a serine-threonine/tyrosine protein kinase catalytic domain that was involved in the abscisic acid (ABA) pathway.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台