FgIlv3a is crucial in branched-chain amino acid biosynthesis, vegetative differentiation, and virulence in Fusarium graminearum
文献类型： 外文期刊
作者： Liu, Xin 1 ; Jiang, Yichen 1 ; Zhang, Yinghui 1 ; Yu, Mingzheng 1 ; Jiang, Hongjun 1 ; Xu, Jianhong 1 ; Shi, Jianrong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, State Key Lab Cultivat Base, Jiangsu Key Lab Food Qual & Safety, Minist Sci & Technol, Nanjing 210014, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Control Technol & Stand Agroprod Safety &, Minist Agr & Rural Affairs, Nanjing 210014, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Key Lab Agroprod Safety Risk Evaluat Nanjing, Minist Agr & Rural Affairs, Nanjing 210014, Jiangsu, Peoples R China
4.Jiangsu Acad Agr Sci, Collaborat Innovat Ctr Modern Grain Circulat & Sa, Nanjing 210014, Jiangsu, Peoples R China
5.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Jiangsu, Peoples R China
6.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
7.Tibet Agr & Anim Husb Univ, Coll Food Sci, Linzhi 860000, Tibet, Peoples R China
8.Xinxiang Med Univ, Coll Life Sci, Sanquan Coll, Xinxiang 453003, Henan, Peoples R China
9.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
关键词： Fusarium graminearum; dihydroxyacid dehydratase; branched-chain amino acid biosynthesis; paralogous FgILV3 genes; virulence
期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）
ISSN： 1225-8873
年卷期： 2019 年 57 卷 8 期
页码：
收录情况： SCI
摘要： Dihydroxyacid dehydratase (DHAD), encoded by ILV3, catalyses the third step in the biosynthetic pathway of branched-chain amino acids (BCAAs), which include isoleucine (Ile), leucine (Leu), and valine (Val). Enzymes involved in BCAA biosynthesis exist in bacteria, plants, and fungi but not in mammals and are therefore attractive targets for antimicrobial or herbicide development. In this study, three paralogous ILV3 genes (FgILV3A, FgILV3B, and FgILV3C) were identified in the genome of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). Deletion of FgILV3A alone or combined with FgILV3B or FgILV3C indicated an important role for FgILV3A in BCAA biosynthesis. FgILV3A deletion mutants lost the ability to grow on medium lacking amino acids. Exogenous supplementation of 1 mM Ile and Val rescued the auxotrophy of Delta FgIlv3A, though 5 mM was required to recover the growth defects in Delta FgIlv3AB and Delta FgIlv3AC strains, indicating that FgIlv3b and FgIlv3c exhibit redundant but accessory roles with FgIlv3a in BCAA biosynthesis. The auxotrophy of Delta FgIlv3A resulted in pleiotropic defects in aerial hyphal growth, in conidial formation and germination, and in aurofusarin accumulation. In addition, the mutants showed reduced virulence and deoxynivalenol production. Overall, our study demonstrates that FgIlv3a is crucial for BCAA biosynthesis in F. graminearum and a candidate fungicide target for FHB management.
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