Differential microRNA Expression in Porcine Endometrium Involved in Remodeling and Angiogenesis That Contributes to Embryonic Implantation
文献类型： 外文期刊
作者： Hong, Linjun 1 ; Liu, Ruize 3 ; Qiao, Xiwu 1 ; Wang, Xingwang 1 ; Wang, Shouqi 1 ; Li, Jiaqi 1 ; Wu, Zhenfang 1 ; Zhang, 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou, Guangdong, Peoples R China
2.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China
3.Broad Inst MIT & Harvard, Stanley Ctr Psychiat Res, Cambridge, MA 02142 USA
关键词： porcine; endometrium; microRNAs; differential expression; implantation
期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）
ISSN： 1664-8021
年卷期： 2019 年 10 卷
页码：
收录情况： SCI
摘要： Background: In western swine breeds, up to 30% of embryonic losses occur during early pregnancy, and the majority of embryonic losses happens during implantation. In this period, maternal recognition of pregnancy begins to occur and blastocysts undergo dramatic morphologic changes. As with other species, changes in the uterine environment plays an important role in the process of embryo implantation in pigs. Erhualian (ER) pigs, one of the Chinese Taihu swine breeds, are known to have the highest litter size in the world. Experiments demonstrated that the greater embryonic survival on gestation day (GD) 12 in Chinese Taihu pigs is one important factor that contributes to enhanced litter size. This is largely controlled by maternal genes. In this study, endometrial samples were collected from pregnant LandracexLarge Yorkshire (LL) sows (parity 3) and ER sows (parity 3) on GD12 and the expression profiles of microRNAs (miRNAs) in the endometrium were compared between ER and LL using miRNA-seq technology. Results: A total of 288 miRNAs were identified in the pig endometrium, including 202 previously known and 86 novel miRNAs. The Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that highly abundant miRNAs might affect endometrial remodeling. Comparison between LL and ER sows revealed that 96 known miRNAs were differentially expressed between the two groups (including 78 up-regulated and 18 down-regulated miRNAs in ER compared to LL). Bioinformatics analysis showed that the target genes of some differentially expressed miRNAs were involved in pathways related to angiogenesis, proliferation, apoptosis, and tissue remodeling, which play critical roles in implantation by regulating endometrial structural changes and secretions of hormones, growth factors, and nutrients. Furthermore, the results demonstrated that insulin-like growth factor-1 protein expression was directly inhibited by miR-206. The lower expression of miR-206 in ER compared to LL might facilitate the angiogenesis of the endometrium during embryo implantation. Conclusions: The identified miRNAs that are differentially expressed in the endometrium of ER and LL pigs will contribute to the understanding of the role of miRNAs in embryonic implantation and the molecular mechanisms of the highest embryonic survival in Chinese ER pigs.
- 相关文献
作者其他论文 更多>>
-
A deep learning-based approach for fully automated segmentation and quantitative analysis of muscle fibers in pig skeletal muscle
作者：Yao, Zekai;Li, Hao;Li, Xinxin;Li, Jianhao;Luo, Yizhi;Meng, Fanming;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Wo, Jingjie;Yin, Ling;Wu, Zhenfang;Luo, Yizhi;Zheng, Enqin;Yang, Jie;Wu, Zhenfang
关键词：Pigs; Skeletal muscle; Deep learning; Image segmentation; Quantitative analysis
-
Multi-omic characterization of allele-specific regulatory variation in hybrid pigs
作者：Quan, Jianping;Wang, Xingwang;Cai, Gengyuan;Ding, Rongrong;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Meng, Fanming;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Lin, Meng;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Chen, Qiaoer;Yang, Jisheng;Gu, Ting;Huang, Sixiu;Xu, Zheng;Li, Zicong;Yang, Jie;Wu, Zhenfang;Quan, Jianping;Ding, Rongrong;Tan, Suxu;Huang, Wen;Quan, Jianping;Cai, Gengyuan;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Lin, Meng;Huang, Sixiu;Yang, Jie;Quan, Jianping;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Yang, Ming;Gao, Xin;Liu, Zhihong;Yang, Ming;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Wang, Xingwang;Cai, Gengyuan;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Gu, Ting;Xu, Zheng;Li, Zicong;Yang, Jie;Meng, Fanming
关键词：
-
Acid phosphatase involved in phosphate homeostasis in Brassica napus and the functional analysis of BnaPAP10s
作者：Zhang, Hao;He, Xuyou;Munyaneza, Venuste;Zhang, Guangzeng;Ye, Xiangsheng;Wang, Chuang;Shi, Lei;Ding, Guangda;Wang, Xu
关键词：Brassica napus; Purple acid phosphatases; Expression profile; BnaPAP10as; Root-associated APase activity; Phosphate homeostasis
-
Protein Dynamic Landscape during Mouse Mammary Gland Development from Virgin to Pregnant, Lactating, and Involuting Stages
作者：Wang, Wenjing;Wang, Shunbo;Wang, Hao;Zheng, Enqin;Wu, Zhenfang;Li, Zicong;Wang, Wenjing;Wang, Shunbo;Wang, Hao;Zheng, Enqin;Wu, Zhenfang;Li, Zicong;Wu, Zhenfang;Li, Zicong
关键词：development; mammary gland; mouse; DIA-based quantitativeproteomics; EGF
-
DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase
作者：Zhou, Chen;Wang, Yongzhong;He, Simin;Lin, Shifei;Cheng, Jie;Hu, Qun;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Zhou, Chen;Wang, Yongzhong;He, Simin;Lin, Shifei;Cheng, Jie;Hu, Qun;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Zhou, Chen;Wang, Yongzhong;He, Simin;Lin, Shifei;Cheng, Jie;Hu, Qun;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Wu, Zhenfang;Wu, Zhenfang;Hong, Linjun
关键词：DIA-based quantitative proteomics; Yorkshire; Endometrium; Peri-implantation; FGL2
-
Protein Dynamic Landscape of Pig Embryos during Peri-Implantation Development
作者：Zang, Xupeng;Huang, Qiuying;Gan, Jianyu;Jiang, Lei;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Zang, Xupeng;Huang, Qiuying;Gan, Jianyu;Jiang, Lei;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun
关键词：development; implantation; peri-implantationembryo; pig; proteome
-
Pig-eRNAdb: a comprehensive enhancer and eRNA dataset of pigs
作者：Wang, Yifei;Pan, Xiangchun;Liao, Weili;Shen, Qingpeng;Cai, Jiali;Gong, Wentao;Tian, Yuhan;Xu, Dantong;Li, Yipeng;Li, Jiaqi;Zhang, Zhe;Yuan, Xiaolong;Jin, Weiwei;Gong, Jing
关键词：
