GPCPD1 is regulated by METTL3/IGF2BP3 dependent m6A modification to affect pig muscle fiber type conversion
文献类型： 外文期刊
作者： Yang, Yuming 1 ; Tan, Baohua 3 ; Xiao, Liyao 1 ; Huang, Huijun 1 ; Xu, Weihua 4 ; Su, Jiawei 1 ; Zhao, Yaolu 5 ; Hong, Linjun 1 ; Cai, Gengyuan 1 ; Li, Zicong 1 ; Dai, Lihe 6 ; Gu, Ting 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou, Peoples R China
2.Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou, Peoples R China
3.Guangzhou Med Univ, Affiliated Hosp 1, Key Lab Adv Interdisciplinary Studies, Guangzhou, Peoples R China
4.Longyan Univ, Coll Life Sci, Longyan, Peoples R China
5.Shenyang Agr Univ, Coll Anim Sci & Vet Med, Key Lab Livestock Infect Dis Northeast China, Minist Educ, Shenyang, Peoples R China
6.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Anim Genet & Breeding Zhejiang Prov, Hangzhou, Peoples R China
关键词： GPCPD1; Pig; m 6 A; Myogenic differentiation; Muscle fiber type conversion
期刊名称：GENE （ 影响因子：2.4； 五年影响因子：2.8 ）
ISSN： 0378-1119
年卷期： 2025 年 962 卷
页码：
收录情况： SCI
摘要： The N6-methyladenosine (m6A) modification is the most prevalent and abundant RNA modification in eukaryotes. In our previous study, we identified that the glycerophosphocholine phosphodiesterase 1 (GPCPD1) gene was differentially expressed and diverse m6A modificated in the pig soleus and extensor digitorum longus muscles. In this study, we further investigated the function of GPCPD1 gene in pig muscle development. We found that GPCPD1 inhibited myogenic differentiation and promoted the conversion of fast-twitch to slow-twitch fibers in both porcine muscle satellite cells (PSCs) and in mouse model. We also found that the expression of GPCPD1 was affected by m6A methyltransferase METTL3. The methylated GPCPD1 gene was recognized by the m6A reader protein IGF2BP3, which further modulated the stability of GPCPD1 mRNA. This study provided novel evidence into m6A regulation in pig muscle development and illustrated the new post-transcriptional regulation way in GPCPD1 expression.
- 相关文献
作者其他论文 更多>>
-
Analysis of differential transcriptome expression reveals that ISG15 provides support for embryo development by promoting angiogenesis in porcine mesometrium
作者：Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Wu, Zhenfang;Hong, Linjun
关键词：Pig; Mesometrium; Transcriptome; Interferon response; ISG15
-
Genome-Wide Association Study Reveals Genetic Mechanisms Underlying Intersex and Aproctia in Large White Pigs
作者：Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Wu, Jie;Yang, Ming;Wu, Zhenfang;Cai, Gengyuan;Wu, Zhenfang;Cai, Gengyuan
关键词：intersex; aproctia; chip data; imputed data; mixed linear models; genome-wide association studies
-
Multi-omic characterization of allele-specific regulatory variation in hybrid pigs
作者：Quan, Jianping;Wang, Xingwang;Cai, Gengyuan;Ding, Rongrong;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Meng, Fanming;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Lin, Meng;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Chen, Qiaoer;Yang, Jisheng;Gu, Ting;Huang, Sixiu;Xu, Zheng;Li, Zicong;Yang, Jie;Wu, Zhenfang;Quan, Jianping;Ding, Rongrong;Tan, Suxu;Huang, Wen;Quan, Jianping;Cai, Gengyuan;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Lin, Meng;Huang, Sixiu;Yang, Jie;Quan, Jianping;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Yang, Ming;Gao, Xin;Liu, Zhihong;Yang, Ming;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Wang, Xingwang;Cai, Gengyuan;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Gu, Ting;Xu, Zheng;Li, Zicong;Yang, Jie;Meng, Fanming
关键词：
-
Protein Dynamic Landscape during Mouse Mammary Gland Development from Virgin to Pregnant, Lactating, and Involuting Stages
作者：Wang, Wenjing;Wang, Shunbo;Wang, Hao;Zheng, Enqin;Wu, Zhenfang;Li, Zicong;Wang, Wenjing;Wang, Shunbo;Wang, Hao;Zheng, Enqin;Wu, Zhenfang;Li, Zicong;Wu, Zhenfang;Li, Zicong
关键词：development; mammary gland; mouse; DIA-based quantitativeproteomics; EGF
-
DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase
作者：Zhou, Chen;Wang, Yongzhong;He, Simin;Lin, Shifei;Cheng, Jie;Hu, Qun;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Zhou, Chen;Wang, Yongzhong;He, Simin;Lin, Shifei;Cheng, Jie;Hu, Qun;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Zhou, Chen;Wang, Yongzhong;He, Simin;Lin, Shifei;Cheng, Jie;Hu, Qun;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Wu, Zhenfang;Wu, Zhenfang;Hong, Linjun
关键词：DIA-based quantitative proteomics; Yorkshire; Endometrium; Peri-implantation; FGL2
-
Protein Dynamic Landscape of Pig Embryos during Peri-Implantation Development
作者：Zang, Xupeng;Huang, Qiuying;Gan, Jianyu;Jiang, Lei;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Zang, Xupeng;Huang, Qiuying;Gan, Jianyu;Jiang, Lei;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun;Gu, Ting;Cai, Gengyuan;Li, Zicong;Wu, Zhenfang;Hong, Linjun
关键词：development; implantation; peri-implantationembryo; pig; proteome
-
The effects of boar seminal plasma extracellular vesicles on sperm fertility
作者：Xu, Zhiqian;Xie, Yanshe;Wu, Changhua;Gu, Ting;Zhang, Xianwei;Yang, Jie;Yang, Huaqiang;Zheng, Enqin;Huang, Sixiu;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Liu, Dewu;Hong, Linjun;Wu, Zhenfang;Xu, Zhiqian;Xie, Yanshe;Wu, Changhua;Gu, Ting;Zhang, Xianwei;Yang, Jie;Yang, Huaqiang;Zheng, Enqin;Huang, Sixiu;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Liu, Dewu;Hong, Linjun;Wu, Zhenfang;Xu, Zhiqian;Wu, Zhenfang;Hong, Linjun;Wu, Zhenfang
关键词：Seminal plasma extracellular vesicles; Proteomics; EZRIN; Sperm; Fertility
