文献类型： 外文期刊
作者： Xu, Zhiqian 1 ; Xie, Yanshe 1 ; Wu, Changhua 1 ; Gu, Ting 1 ; Zhang, Xianwei 1 ; Yang, Jie 1 ; Yang, Huaqiang 1 ; Zheng, Enqin 1 ; Huang, Sixiu 1 ; Xu, Zheng 1 ; Li, Zicong 1 ; Cai, Gengyuan 1 ; Liu, Dewu 1 ; Hong, Linjun 1 ; Wu, Zhenfang 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
2.Lingnan Guangdong Lab Modern Agr, Guangzhou 510642, Guangdong, Peoples R China
3.Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471023, Henan, Peoples R China
4.Wens Foodstuff Grp Co Ltd, Yunfu 527400, Guangdong, Peoples R China
5.Coll Anim Sci, 483 wushan Rd, Guangzhou, Guangdong, Peoples R China
关键词： Seminal plasma extracellular vesicles; Proteomics; EZRIN; Sperm; Fertility
期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）
ISSN： 0093-691X
年卷期： 2024 年 213 卷
页码：
收录情况： SCI
摘要： Extracellular vesicles (EVs) are abundant in body fluid and are critical in cell interaction. Seminal plasma contains numerous EVs which affecting sperm function via transferring regulatory cargoes to the sperm. However, the mechanism of seminal plasma extracellular vesicles (SP-EVs) is still not clear. The present study aimed to isolate the boar SP-EVs and explore its potential function, then identify the key protein involved in SP-EVs and sperms interaction, and elucidate mechanism of SP-EVs protein on sperms. Here, we successfully isolated and concentrated boar SP-EVs, the SP-EVs showed a typical vesicle structure under transmission electron microscopy, most of their diameters range between 50 and 200 nm and express EVs biomarkers CD9 and CD63. We proved that SP-EVs could inhibit sperm acrosome reaction and in vitro fertility. Through a data-independent acquisition analysis of protein profiles of noncapacitated sperms, normal capacitated sperms and SP-EVs treated capacitated sperms, we identified that EZRIN was one of the active proteins that participated in SP-EVs and sperms interaction. Furthermore, we tested that the inhibition of EZRIN could promote boar sperm fertility, which is in consistence with the function of SP-EVs. The results may facilitate future research of SP-EVs on sperm function and male infertility.
- 相关文献
作者其他论文 更多>>
-
RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs
作者：Xiao, Liyao;Qiao, Jiaxin;Huang, Yiyang;Tan, Baohua;Hong, Linjun;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Zheng, Enqin;Wang, Shanshan;Gu, Ting;Li, Zicong;Wu, Zhenfang;Li, Zicong;Wu, Zhenfang;Li, Zicong;Wu, Zhenfang;Cai, Gengyuan;Wu, Zhenfang;Wang, Shanshan
关键词：RASGRP1; H3K27me3; cell proliferation; myoblasts; skeletal muscle
-
Genome-Wide Identification and Expression Analysis of the Class III Peroxidase Gene Family under Abiotic Stresses in Litchi (Litchi chinensis Sonn.)
作者：Yang, Jie;Chen, Rong;Xiang, Xu;Liu, Wei;Fan, Chao
关键词：litchi; PRX gene family; phylogenetic tree; abiotic stresses; expression pattern
-
Genome-wide detection of multiple variants associated with teat number in French Yorkshire pigs
作者：Lin, Danyang;Qiu, Yibin;Zhou, Fuchen;Li, Xuehua;Deng, Shaoxiong;Yang, Jisheng;Chen, Qiaoer;Cai, Gengyuan;Yang, Jie;Wu, Zhenfang;Zheng, Enqin;Lin, Danyang;Qiu, Yibin;Zhou, Fuchen;Li, Xuehua;Deng, Shaoxiong;Yang, Jisheng;Chen, Qiaoer;Cai, Gengyuan;Yang, Jie;Wu, Zhenfang;Zheng, Enqin;Cai, Gengyuan;Wu, Zhenfang;Cai, Gengyuan;Yang, Jie;Wu, Zhenfang;Wu, Zhenfang
关键词：Teat number; Copy number variation; Genome-wide association study; French Yorkshire pigs
-
Identification and Comprehensive Analysis of OFP Genes for Fruit Shape Influence in Mango
作者：Wu, Qiuping;Yang, Jie;Chen, Rong;Fan, Chao;Xia, Rui;Zeng, Zaohai
关键词：mango (Mangifera indica L.); OVATE family proteins; expression pattern; fruit development
-
Dynamic intrauterine crosstalk promotes porcine embryo implantation during early pregnancy
作者：Zang, Xupeng;Gu, Shengchen;Wang, Wenjing;Gan, Jianyu;Hu, Qun;Zhou, Chen;Ding, Yue;He, Yanjuan;Jiang, Lei;Gu, Ting;Xu, Zheng;Huang, Sixiu;Yang, Huaqiang;Li, Zicong;Cai, Gengyuan;Hong, Linjun;Wu, Zhenfang;Zang, Xupeng;Gu, Shengchen;Wang, Wenjing;Gan, Jianyu;Hu, Qun;Zhou, Chen;Ding, Yue;He, Yanjuan;Gu, Ting;Xu, Zheng;Huang, Sixiu;Yang, Huaqiang;Li, Zicong;Cai, Gengyuan;Hong, Linjun;Wu, Zhenfang;Shi, Junsong;Wu, Zhenfang;Meng, Fanming;Gu, Ting;Xu, Zheng;Huang, Sixiu;Yang, Huaqiang;Li, Zicong;Cai, Gengyuan;Hong, Linjun;Wu, Zhenfang
关键词：endometrial remodeling; ExtraCellTalk; implantation; maternal-foetal crosstalk; trophoblast
-
Genome-Wide Characterization and Phylogenetic and Stress Response Expression Analysis of the MADS-Box Gene Family in Litchi (Litchi chinensis Sonn.)
作者：Yang, Jie;Chen, Rong;Liu, Wei;Xiang, Xu;Fan, Chao
关键词：litchi; MADS-box gene family; phylogenetic analysis; stress response; gene expression
-
Transcriptome analysis of mRNA and miRNA in the development of LeiZhou goat muscles
作者：Fu, Junjie;Liu, Jie;Deng, Ming;Liu, Guangbin;Sun, Baoli;Guo, Yongqing;Liu, Dewu;Li, Yaokun;Liu, Jie;Zou, Xian;Fu, Junjie;Liu, Jie;Deng, Ming;Liu, Guangbin;Sun, Baoli;Guo, Yongqing;Liu, Dewu
关键词：
