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DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase

文献类型： 外文期刊

作者： Zhou, Chen 1 ; Wang, Yongzhong 1 ; He, Simin 1 ; Lin, Shifei 1 ; Cheng, Jie 1 ; Hu, Qun 1 ; Meng, Fanming 4 ; Gu, Ting 1 ; Cai, Gengyuan 1 ; Li, Zicong 1 ; Wu, Zhenfang 1 ; Hong, Linjun 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, State Key Lab Swine & Poultry Breeding Ind, Guangzhou, Peoples R China

2.Natl Engn Res Ctr Breeding Swine Ind, Guangzhou, Peoples R China

3.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou, Peoples R China

4.Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou, Peoples R China

5.Yunfu Subctr Guangdong Lab Lingnan Modern Agr, Yunfu, Peoples R China

6.Minist Agr & Rural Affairs, Key Lab South China Modern Biol Seed Ind, Guangzhou, Peoples R China

关键词： DIA-based quantitative proteomics; Yorkshire; Endometrium; Peri-implantation; FGL2

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 1874-3919

年卷期： 2024 年 293 卷

页码：

收录情况： SCI

摘要： The 12th day of gestation is a critical period for embryo loss and the beginning of imminent implantation in sows. Data independent acquisition (DIA) technology is one of the high-throughput, high-resolution and reproducible proteomics technologies for large-scale digital qualitative and quantitative research. The aim of this study was to identify and characterize the protein abundance landscape of Yorkshire pig endometrium on the 12th day of pregnancy (P12) and estrous cycle (C12) using DIA proteomics. A total of 1251 differentially abundant proteins (DAPs) were identified, of which 882 were up-regulated and 369 were down-regulated at P12. Functional enrichment analysis showed that the identified proteins were related to metabolism, biosynthesis and signaling pathways. Three proteins were selected for Western blot (WB) validation and the results were consistent with the DIA data. Further combined with transcriptome data, fibrinogen like 2 (FGL2) and S100 calcium binding protein A8 (S100A8) were verified to be highly abundant in the P12 endometrial epithelium. In summary, there were significantly different abundance of proteome profiles in C12 and P12 endometrium, suggesting that DAPs are associated with changes in endometrial receptivity, which laid the foundation for further research on related regulatory mechanisms. Significance: The 12th day of gestation is an important point in the peri-implantation period of pigs, when the endometrium presents a receptive state under the stimulation of estrogen. DIA proteomics technology is an emerging protein identification technology in recent years, which can obtain protein information through comprehensive and unbiased scanning. In this study, DIA technology was used to characterize endometrial proteins in pigs during the peri-implantation period. The results showed that higher protein abundance was detected using the DIA technique, and some of these DAPs may be involved in regulating embryo implantation. This study will help to better reveal the related proteins involved in embryo implantation, and lay a foundation for further research on the mechanism of endometrial regulation of embryo implantation. Significance of the study: The 12th day of gestation is an important point in the peri-implantation period of pigs, when the endometrium presents a receptive state under the stimulation of estrogen. DIA proteomics technology is an emerging protein identification technology in recent years, which can obtain protein information through comprehensive and unbiased scanning. In this study, DIA technology was used to characterize endometrial proteins in pigs during the peri-implantation period. The results showed that higher protein abundance was detected using the DIA technique, and some of these DAPs may be involved in regulating embryo implantation. This study will help to better reveal the related proteins involved in embryo implantation, and lay a foundation for further research on the mechanism of endometrial regulation of embryo implantation.

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