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A dual signal immunochromatographic strip for the detection of imidaclothiz using a recombinant fluorescent-peptide tracer and gold nanoparticles

文献类型： 外文期刊

作者： Ding, Yuan 1 ; Hua, Xiude 1 ; Chen, He 1 ; Gonzalez-Sapienza, Gualberto 2 ; Barnych, Bogdan 3 ; Liu, Fengquan 1 ; Wang 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Univ Republica, Inst Higiene, Fac Quim, Catedra Inmunol, Montevideo 11600, Uruguay

3.Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA

4.Univ Calif Davis, UCD Canc Ctr, Davis, CA 95616 USA

5.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词： Sensitivity; Fluorescent peptide tracer; Inner filter effect; Dual signals; Pesticide residue

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN：

年卷期： 2019 年 297 卷

页码：

收录情况： SCI

摘要： Immunochromatographic strips (ICS) attract much attention as practical analytical tools, particularly for point of care testing. However, ICS suffer from insufficient sensitivity, especially for small molecules. Competitive assays developed with analyte peptidomimetics obtained from phage display peptide libraries, usually enable to attain lower detection limits than the widely used chemical haptens, but are rarely used in ICS assay because of the shortcomings of the phage particle. In this work, we used a phage-free fluorescent peptide tracer (C2-15-EmGFP) for ICS, which consists of the emerald green fluorescent protein (EmGFP) fused to a peptidomimetic of imidaclothiz (C2-15). C2-15-EmGFP works as fluorescent donor and colloidal gold nanoparticles (AuNPs) coated with an anti-imidaclohtiz antibody as quencher, which allowed to develop a fluorescence quenching-based ICS (FQICS) for the detection of imidaclothiz in agricultural and environmental samples. The colorimetric and fluorescent signals were determined by both naked eye and imageJ software. Quantitative image analysis showed that the sensitivities obtained using the colorimetric (50% inhibitory concentration (IC50): 9.62 ng mL(-1)) and fluorescent (50% saturation concentration (SC50): 10.1 ng mL(-1)) signals were similar. The visual detection by the naked eye showed a sensitivity of 8.00 ng mL(-1) with fluorescent readout which was 8-fold lower than that of the colorimetric readout (64.0 ng mL(-1)). The FQICS performed with excellent recoveries and good correlation with high-performance liquid chromatography in different matrices.

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