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A feed-forward signalling circuit controls bacterial virulence through linking cyclic di-GMP and two mechanistically distinct sRNAs, ArcZ and RsmB

文献类型： 外文期刊

作者： Yuan, Xiaochen 2 ; Zeng, Quan 3 ; Khokhani, Devanshi 2 ; Tian, Fang 4 ; Severin, Geoffrey B. 5 ; Waters, Christophe 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Jiangsu, Peoples R China

2.Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA

3.Connecticut Agr Expt Stn, Dept Plant Pathol & Ecol, New Haven, CT 06511 USA

4.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

5.Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

6.Zhejiang Agr & Forestry Univ, Sch Forestry & Biotechnol, Hangzhou 311300, Zhejiang, Peoples R China

7.Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA

8.ARS, US Salin Lab, USDA, Riverside, CA 92507 USA

期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.491； 五年影响因子：6.438 ）

ISSN： 1462-2912

年卷期： 2019 年 21 卷 8 期

页码：

收录情况： SCI

摘要： Dickeya dadantii is a plant pathogen that causes soft rot disease on vegetable and potato crops. To successfully cause infection, this pathogen needs to coordinately modulate the expression of genes encoding several virulence determinants, including plant cell wall degrading enzymes (PCWDEs), type III secretion system (T3SS) and flagellar motility. Here, we uncover a novel feed-forward signalling circuit for controlling virulence. Global RNA chaperone Hfq interacts with an Hfq-dependent sRNA ArcZ and represses the translation of pecT, encoding a LysR-type transcriptional regulator. We demonstrate that the ability of ArcZ to be processed to a 50 nt 3 '- end fragment is essential for its regulation of pecT. PecT down-regulates PCWDE and the T3SS by repressing the expression of a global post-transcriptional regulator- (RsmA-) associated sRNA encoding gene rsmB. In addition, we show that the protein levels of two cyclic di-GMP (c-di-GMP) diguanylate cyclases (DGCs), GcpA and GcpL, are repressed by Hfq. Further studies show that both DGCs are essential for the Hfq-mediated post-transcriptional regulation on RsmB. Overall, our report provides new insights into the interplays between ubiquitous signalling transduction systems that were most studied independently and sheds light on multitiered regulatory mechanisms for a precise disease regulation in bacteria.

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