The casein kinase MoYck1 regulates development, autophagy, and virulence in the rice blast fungus
文献类型： 外文期刊
作者： Shi, Huan-Bin 1 ; Chen, Nan 1 ; Zhu, Xue-Ming 1 ; Su, Zhen-Zhu 1 ; Wang, Jiao-Yu 3 ; Lu, Jian-Ping 4 ; Liu, Xiao-Hong; 1 ;
作者机构： 1.Zhejiang Univ, Biotechnol Inst, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China
2.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Plant Protect Microbiol, State Key Lab Qual & Safety Agroprod, Hangzhou, Zhejiang, Peoples R China
4.Zhejiang Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China
关键词： Magnaporthe oryzae; casein kinase; development; autophagy; virulence
期刊名称：VIRULENCE （ 影响因子：5.882； 五年影响因子：6.489 ）
ISSN： 2150-5594
年卷期： 2019 年 10 卷 1 期
页码：
收录情况： SCI
摘要： Casein kinases are serine/threonine protein kinases that are evolutionarily conserved in yeast and humans and are involved in a range of important cellular processes. However, the biological functions of casein kinases in the fungus Magnaporthe oryzae, the causal agent of destructive rice blast disease, are not characterized. Here, two casein kinases, MoYCK1 and MoHRR25, were identified and targeted for replacement, but only MoYCK1 was further characterized due to the possible nonviability of the MoHRR25 deletion mutant. Disruption of MoYCK1 caused pleiotropic defects in growth, conidiation, conidial germination, and appressorium formation and penetration, therefore resulting in reduced virulence in rice seedlings and barley leaves. Notably, the MoYCK1 deletion triggered quick lipidation of MoAtg8 and degradation of the autophagic marker protein GFP-MoAtg8 under nitrogen starvation conditions, in contrast to the wild type, indicating that autophagy activity was negatively regulated by MoYck1. Furthermore, we found that HOPS (homotypic fusion and vacuolar protein sorting) subunit MoVps41, a putative substrate of MoYck1, was co-located with MoAtg8 and positively required for the degradation of MoAtg8-PE and GFP-MoAtg8. In addition, MoYCK1 is also involved in the response to ionic hyperosmotic and heavy metal cation stresses. Taken together, our results revealed crucial roles of the casein kinase MoYck1 in regulating development, autophagy and virulence in M. oryzae.
- 相关文献
作者其他论文 更多>>
-
The endosomal-vacuolar transport system acts as a docking platform for the Pmk1 MAP kinase signaling pathway in Magnaporthe oryzae
作者：Wang, Qing;Huang, Zhicheng;Li, Yan;Li, Hui;Lin, Fu-Cheng;Lu, Jianping;Wang, Jing;Cai, Yingying;Wang, Jiaoyu;Lin, Fu-Cheng;Huang, Pengyun;Liu, Xiaohong;Lin, Fu-Cheng
关键词：appressorium; endosome; MAPK; Pmk1; rice blast fungus; signal; ubiquitination; vacuole
-
A multilayered regulatory network mediated by protein phosphatase 4 controls carbon catabolite repression and de-repression in Magnaporthe oryzae
作者：Huang, Zhicheng;Wang, Qing;Li, Yan;Li, Hui;Lin, Fu-Cheng;Lu, Jianping;Huang, Pengyun;Liao, Jian;Liu, Xiaohong;Lin, Fu-Cheng;Wang, Jing;Cai, Yingying;Wang, Jiaoyu;Lin, Fu-Cheng
关键词：
-
MoSec13 combined with MoGcn5b modulates MoAtg8 acetylation and regulates autophagy in Magnaporthe oryzae
作者：Qian, Hui;Wu, Ming-Hua;Zhao, Wen-Hui;Lin, Fu-Cheng;Liu, Xiao-Hong;Zhu, Xue-Ming;Lin, Fu-Cheng;Zhu, Xue-Ming;Lin, Fu-Cheng;Sun, Li-Xiao;Lu, Jian-Ping;Klionsky, Daniel J.
关键词：Autophagy; Gcn5b;
Magnaporthe oryzae ; Sec13; TORC2 -
The whole genome sequence of Cordyceps cicadae - an edible and potential medicinal fungus
作者：Liu, Yuwei;Li, Xueqian;Meng, Yiqi;Zhou, Wei;Lin, Fu-Cheng;Wang, Hongkai;Lin, Fu-Cheng;Wu, Yifan;Jin, Yuting;Ma, Xiaotong;Tan, Yuchong
关键词：
Cordyceps cicadae ; Whole genome; Hi-C sequencing -
FK506 Targets MoFpr1 to Modulate Autophagy and Ubiquitination, Inhibiting the Pathogenicity of Magnaporthe oryzae
作者：Zhao, Wen-Hui;Yan, Jiong-Yi;Xie, Ke-Xin;Luo, Xi-Chun;Liao, Jian;An, Chun-Yue;Liu, Xiao-Hong;Yan, Jiong-Yi;Liang, Shuang;Zhu, Xue-Ming;Lin, Fu-Cheng;Wu, Xi-Yu;Qian, Hui;Lu, Jian-Ping;Khan, Irshad Ali
关键词：FK506; X-ray crystallography; riceblast; broad-spectrum antifungal activity
-
Nuclear basket nucleoporin MoNup50 is essential for fungal development, pathogenicity, and autophagy in Magnaporthe oryzae
作者：Cai, Ying-Ying;Zhu, Xue-Ming;Noman, Muhammad;Wang, Jing;Hao, Zhong-Na;Wang, Yan-Li;Li, Lin;Wang, Jiao-Yu;Lin, Fu-Cheng;Liu, Xiao-Hong;Lin, Fu-Cheng;Lu, Jian-Ping
关键词：Rice blast fungus; Virulence; Atg7; Nuclear pore complex
-
A rho-type GTPase activating protein affects the growth and development of Cordyceps cicadae
作者：Li, Xueqian;Zou, Yu;Wang, Hongkai;Shrivastava, Neeraj;Bao, Jiandong;Lin, Fu-Cheng
关键词：Cordyceps cicadae; Rho-type GTPase activating protein; Growth and development; Active components
