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Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

文献类型： 外文期刊

作者： Zhan, Haixia 1 ; Li, Du 1 ; Dewer, Youssef 3 ; Niu, Changying 2 ; Li, Fengqi 1 ; Luo, Chen 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing Key Lab Environm Friendly Management Frui, Beijing, Peoples R China

2.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Key Lab Insect Resource Applicat & Sustaina, Wuhan, Peoples R China

3.Agr Res Ctr, Cent Agr Pesticide Lab, Bioassay Res Dept, Giza, Egypt

关键词： Drosophila suzukii; Odorant-binding proteins; Competitive binding; cis-vaccenyl acetate (cVA); (Z)-7-tricosene; beta-ionone; Berberine

期刊名称：HELIYON

ISSN：

年卷期： 2021 年 7 卷 3 期

页码：

收录情况： SCI

摘要： The fruit fly Drosophila suzukii is a fruit crop pest that causes a severe economic threat to soft summer fruit worldwide. The male sex pheromone, cis-vaccenyl acetate (cVA) has multiple functions in intra-species communication in Drosophila melanogaster, which is required in male to suppress male-male courtship. D. suzukii males do not produce cVA; however, the odorant receptor for cVA (Or67d) is still functional. The lack of cVA in D. suzukii casts the question of whether this pheromone might have been replaced by another compound similar to cVA that disrupts mating in D. suzukii. In order to address this question, we cloned two D. suzukii adult antenna-specific odorant-binding proteins (OBPs) DsOBP69a and DsOBP76a and aligned with their D. melanogaster orthologues. Moreover, we examined the binding properties of the newly identified recombinant proteins against 26 potential ligands including cVA, using the fluorescence-based ligand binding assay. The alignment showed that DsOBP69a and DsOBP76a, have six conserved cysteines and belong to the classic OBP family. Furthermore, our results revealed that cVA did not bind to DsOBP69a or DsOBP76a proteins. Interestingly, the floral odorant beta-ionone and the bitter substance berberine chloride and coumarin displayed high binding ability. It is also worth noting that DsOBP69a and DsOBP76a have different affinities to (Z)-7-Tricosene that may reflect different functional roles. These findings suggest that DsOBP69a and DsOBP76a are potentially involved in olfaction and gustation of D. suzukii.

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