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RNA interference shows that Spook, the precursor gene of 20-hydroxyec-dysone (20E), regulates the molting of Macrobrachium nipponense

文献类型： 外文期刊

作者： Yuan, Huwei 1 ; Qiao, Hui 2 ; Fu, Yin 3 ; Fu, Hongtuo 1 ; Zhang, Wenyi 2 ; Jin, Shubo 2 ; Gong, Yongsheng 2 ; Jiang, Sufe 1 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Jiangsu, Peoples R China

3.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

关键词： Macrobrachium nipponense; Mn-Spook; 20E; RNA interference; Molt

期刊名称：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.294； 五年影响因子：4.217 ）

ISSN： 0960-0760

年卷期： 2021 年 213 卷

页码：

收录情况： SCI

摘要： The aim of this study was to explore the function of the Mn-Spook gene, which was found in the ovary transcriptome of the Oriental river prawn (Macrobrachium nipponense). The Spook gene, which is the precursor gene of 20-hydroxyecdysone (20E), plays an important role in the process of molting in many arthropods, but its function in M. nipponense is unclear. We cloned the full-length Mn-Spook gene from the ovary of M. nipponense and found that it had the same conserved domains as the P450 gene of the Halloween family of genes. The MnSpook gene was highly expressed in ovary and gill tissue during the breeding period. During ovarian development, Mn-spook gene expression was highest at the nearly-ripe stage, and it also was highly expressed in the zoea developmental stage. Cellular localization analysis showed that Mn-Spook signals accumulated in the cytoplasmic membrane and nucleus of oocytes. Finally, we used RNA interference to evaluate the function of the Mn-Spook gene. Compared with the control group, in vivo injection of Mn-Spook dsRNA effectively downregulated the expression of Mn-Spook and the content of 20E. The molting frequency of M. nipponense in the experimental group also was significantly inhibited. These results demonstrated that the Mn-Spook gene played an important role in the molting process of M. nipponense.

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